The final publication is available via <a href="https://doi.org/10.1039/c4py00792a" target="_blank">https://doi.org/10.1039/c4py00792a</a>.
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dc.description.abstract
Two-photon polymerization (2PP) allows 3D microfabrication of biomaterial scaffolds with user-defined geometry. This technique is highly promising for 3D cell culture and tissue engineering. However, biological applications of 2PP require photopolymerizable hydrogels with high reactivity and low cytotoxicity. This paper describes a novel hydrogel system based on hyaluronic acid vinyl esters (HA-VE), which enabled fast 2PP-fabrication of 3D hydrogel constructs with μm-scale accuracy. A series of HA-VE macromers with tunable degrees of substitution were synthesized by lipasecatalyzed transesterification. HA-VE gels were proved to be injectable, photocurable, enzymatically degradable and mechanically comparable to various soft tissues. Owing to the unique molecular design, degradation products of HA-VE gels through hydrolysis are non-toxic polyvinyl alcohol and adipic acid. Furthermore, HA-VE gels were systematically characterized and compared to HA-acrylates (HA-AC) and HA-methacrylates (HA-MA) gels including macromer cytotoxicity, photoreactivity, swelling, and gel stiffness. Cytotoxicity assay with L929 fibroblasts revealed that HA-VE was significantly less toxic than HA-AC (P<0.01) and HA-MA (P<0.05). Crosslinking efficiency of HA-VE was comparable to HA-AC and much higher than HA-MA. Although the reactivity of HA-VE for homopolymerization was insufficient for 2PP, it was demonstrated that thiol-ene chemistry could substantially improve its reactivity. This optimization led to 2PP-fabrication of a HA-VE hydrogel construct with μm-scale accuracy. Low cytotoxicity, high reactivity and good biodegradability makes HA-VE promising candidates for biological applications in cell culture and tissue engineering.
en
dc.language
English
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dc.language.iso
en
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dc.publisher
Royal Society of Chemistry
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dc.relation.ispartof
Polymer Chemistry
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dc.rights.uri
http://rightsstatements.org/vocab/InC/1.0/
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dc.title
Enzymatic synthesis of hyaluronic acid vinyl esters for two-photon microfabrication of biocompatible and biodegradable hydrogel constructs
en
dc.type
Article
en
dc.type
Artikel
de
dc.rights.license
Urheberrechtsschutz
de
dc.rights.license
In Copyright
en
dc.contributor.affiliation
TU Wien, Österreich
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dc.rights.holder
The Royal Society of Chemistry 2014
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dc.type.category
Original Research Article
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tuw.journal.peerreviewed
true
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tuw.peerreviewed
true
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tuw.version
am
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dcterms.isPartOf.title
Polymer Chemistry
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tuw.publication.orgunit
E308 - Institut für Werkstoffwissenschaft und Werkstofftechnologie
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tuw.publisher.doi
10.1039/c4py00792a
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dc.identifier.eissn
1759-9962
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dc.identifier.libraryid
AC11359638
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dc.identifier.urn
urn:nbn:at:at-ubtuw:3-1115
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tuw.author.orcid
0000-0001-8984-5672
-
tuw.author.orcid
0000-0003-2733-9947
-
tuw.author.orcid
0000-0002-3626-5647
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tuw.author.orcid
0000-0001-5846-0198
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tuw.author.orcid
0000-0001-7865-1936
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dc.rights.identifier
Urheberrechtsschutz
de
dc.rights.identifier
In Copyright
en
wb.sci
true
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item.openaccessfulltext
Open Access
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item.openairecristype
http://purl.org/coar/resource_type/c_2df8fbb1
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item.grantfulltext
open
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item.languageiso639-1
en
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item.openairetype
research article
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item.fulltext
with Fulltext
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item.cerifentitytype
Publications
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crisitem.author.dept
TU Wien
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crisitem.author.dept
E308 - Institut für Werkstoffwissenschaft und Werkstofftechnologie
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crisitem.author.dept
E308-50-2 - Fachgruppe Technische Assistenz
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crisitem.author.dept
E134 - Institut für Angewandte Physik
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crisitem.author.dept
E134 - Institut für Angewandte Physik
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crisitem.author.dept
E308-02 - Forschungsbereich Polymer- und Verbundwerkstoffe
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crisitem.author.dept
E308-02-3 - Forschungsgruppe 3D Printing and Biofabrication