<div class="csl-bib-body">
<div class="csl-entry">Bäumgen, M., Dutschei, T., Bartosik, D., Suster, C., Reisky, L., Gerlach, N., Stanetty, C., Mihovilovic, M., Schweder, T., Hehemann, J.-H., & Bornscheuer, U. T. (2021). A new carbohydrate-active oligosaccharide dehydratase is involved in the degradation of ulvan. <i>Journal of Biological Chemistry</i>, <i>297</i>(4), 101210. https://doi.org/10.1016/j.jbc.2021.101210</div>
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dc.identifier.issn
0021-9258
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/137862
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dc.description.abstract
Marine algae catalyze half of all global photosynthetic production of carbohydrates. Owing to their fast growth rates, Ulva spp. rapidly produce substantial amounts of carbohydrate-rich biomass and represent an emerging renewable energy and carbon resource. Their major cell wall polysaccharide is the anionic carbohydrate ulvan. Here, we describe a new enzymatic degradation pathway of the marine bacterium Formosa agariphila for ulvan oligosaccharides involving unsaturated uronic acid at the non-reducing end linked to rhamnose-3-sulfate and glucuronic or iduronic acid (Δ-Rha3S-GlcA/IdoA-Rha3S). Notably, we discovered a new dehydratase (P29_PDnc) acting on the non-reducing end of ulvan oligosaccharides, i.e. GlcA/IdoA-Rha3S, forming the aforementioned unsaturated uronic acid residue. This residue represents the substrate for GH105 glycoside hydrolases, which complements the enzymatic degradation pathway including one ulvan lyase, one multimodular sulfatase, three glycoside hydrolases and the dehydratase P29_PDnc, the latter being described for the first time. Our research thus shows that the oligosaccharide dehydratase is involved in the degradation of carboxylated polysaccharides into monosaccharides.
en
dc.language.iso
en
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dc.relation.ispartof
Journal of Biological Chemistry
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dc.subject
Biochemistry
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dc.subject
Molecular Biology
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dc.subject
Cell Biology
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dc.subject
pathway elucidation
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dc.subject
carbohydrate-active anzymes
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dc.subject
novel enzyme
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dc.subject
dehydratase
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dc.subject
marine polysaccharide
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dc.subject
ulvan
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dc.subject
enzyme mechanism
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dc.subject
sulfatase
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dc.subject
glycoside hydrolase
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dc.title
A new carbohydrate-active oligosaccharide dehydratase is involved in the degradation of ulvan