<div class="csl-bib-body">
<div class="csl-entry">Schnichels, S., Schultheiss, M., Klemm, P., Blak, M., Herrmann, T., Melchinger, M., Bartz-Schmidt, K.-U., Löscher, M., Zeck, G. M., Spitzer, M. S., & Hurst, J. (2021). Cyclosporine A Protects Retinal Explants against Hypoxia. <i>International Journal of Molecular Sciences</i>, <i>22</i>(19), 10196. https://doi.org/10.3390/ijms221910196</div>
</div>
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dc.identifier.issn
1661-6596
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/138929
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dc.description.abstract
The retina is a complex neurological tissue and is extremely sensitive to an insufficient supply of oxygen. Hypoxia plays a major role in several retinal diseases, and often results in the loss of cells that are essential for vision. Cyclosporine A (CsA) is a widely used immunosuppressive drug. Furthermore, treatment with CsA has neuroprotective effects in several neurologic disorders. No data are currently available on the tolerated concentration of CsA when applied to the retina. To reveal the most effective dose, retinal explants from rat eyes were exposed to different CsA concentrations (1-9 µg/mL). Immunohistochemistry with brain-specific homeobox/POU domain protein 3a (Brn3a) and TUNEL staining was performed to determine the percentage of total and apoptotic retinal ganglion cells (RGCs), as well as the responses of micro- and macroglial cells. Furthermore, optical coherence tomography (OCT) scans were performed to measure the changes in retinal thickness, and recordings with multielectrode array (MEA) were performed to evaluate spontaneous RGC spiking. To examine the neuroprotective effects, retinas were subjected to a hypoxic insult by placing them in a nitrogen-streamed hypoxic chamber prior to CsA treatment. In the biocompatibility tests, the different CsA concentrations had no negative effect on RGCs and microglia. Neuroprotective effects after a hypoxic insult on RGCs was demonstrated at a concentration of 9 µg/mL CsA. CsA counteracted the hypoxia-induced loss of RGCs, reduced the percentage of TUNEL+ RGCs, and prevented a decrease in retinal thickness. Taken together, the results of this study suggest that CsA can effectively protect RGCs from hypoxia, and the administered concentrations were well tolerated. Further in vivo studies are needed to determine whether local CsA treatment may be a suitable option for hypoxic retinal diseases.
en
dc.language.iso
en
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dc.relation.ispartof
International Journal of Molecular Sciences
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dc.subject
Computer Science Applications
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dc.subject
General Medicine
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dc.subject
Inorganic Chemistry
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dc.subject
Spectroscopy
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dc.subject
Catalysis
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dc.subject
Molecular Biology
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dc.subject
Physical and Theoretical Chemistry
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dc.subject
Organic Chemistry
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dc.subject
hypoxia
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dc.subject
ischemia
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dc.subject
cyclosporine A
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dc.subject
retinal ganglion cells
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dc.subject
neuroprotection
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dc.title
Cyclosporine A Protects Retinal Explants against Hypoxia
en
dc.type
Artikel
de
dc.type
Article
en
dc.contributor.affiliation
University of Tübingen
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dc.description.startpage
10196
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dc.type.category
Original Research Article
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tuw.container.volume
22
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tuw.container.issue
19
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tuw.journal.peerreviewed
true
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tuw.peerreviewed
true
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wb.publication.intCoWork
International Co-publication
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tuw.researchTopic.id
M6
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tuw.researchTopic.id
I8
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tuw.researchTopic.id
X1
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tuw.researchTopic.name
Biological and Bioactive Materials
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tuw.researchTopic.name
Sensor Systems
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tuw.researchTopic.name
außerhalb der gesamtuniversitären Forschungsschwerpunkte
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tuw.researchTopic.value
50
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tuw.researchTopic.value
30
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tuw.researchTopic.value
20
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dcterms.isPartOf.title
International Journal of Molecular Sciences
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tuw.publication.orgunit
E363 - Institut für Biomedizinische Elektronik
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tuw.publisher.doi
10.3390/ijms221910196
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dc.identifier.eissn
1422-0067
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dc.description.numberOfPages
1
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tuw.author.orcid
0000-0002-4736-2274
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tuw.author.orcid
0000-0002-4588-1767
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tuw.author.orcid
0000-0001-9264-5145
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tuw.author.orcid
0000-0003-3998-9883
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wb.sci
true
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wb.sciencebranch
Elektrotechnik, Elektronik, Informationstechnik
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wb.sciencebranch
Biologie
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wb.sciencebranch.oefos
2020
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wb.sciencebranch.oefos
1060
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wb.facultyfocus
Außerhalb der primären Forschungsgebiete der Fakultät
de
wb.facultyfocus
Outside the Faculty's primary research activities
en
item.languageiso639-1
en
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item.cerifentitytype
Publications
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Publications
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Artikel
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Article
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http://purl.org/coar/resource_type/c_18cf
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http://purl.org/coar/resource_type/c_18cf
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item.fulltext
no Fulltext
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item.grantfulltext
none
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crisitem.author.dept
University of Tübingen
-
crisitem.author.dept
E363 - Institut für Biomedizinische Elektronik
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0000-0002-4736-2274
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crisitem.author.orcid
0000-0001-9264-5145
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0000-0003-3998-9883
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E350 - Fakultät für Elektrotechnik und Informationstechnik