<div class="csl-bib-body">
<div class="csl-entry">Soleymani, N., Birner-Grünberger, R., Abnous, K., Borji, H., & Vahdati Hassani, F. (2021). Identification and Immunological Characterization of Somatic Proteins from Adults of Toxocara cati by Proteomics Technique. <i>Iranian Journal of Parasitology</i>. https://doi.org/10.18502/ijpa.v16i1.5508</div>
</div>
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dc.identifier.issn
1735-7020
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/139026
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dc.description.abstract
Background: Toxocara cati is considered as one of the main etiological agents of toxocariasis with global and regional importance. As there is no information on proteomics of T. cati, herein, we reported the results obtained by proteomic analysis of somatic proteins extract, using a mass spectrometry (LC-MS/MS) approach.
Methods: Somatic extract fractions were separated by two-dimensional SDS-PAGE and were electro blotted on to PVDF membranes for immunoblot analysis, then collected the immunogenic spots which response of antibodies of the paratenic hosts (mice) to the antigens (Mashhad, 2017), and analyzed by LC-MS/MS. The LC-MS/MS data were analyzed by Mascot database, Taxonomy Toxocara, and common contaminants, in Omics Center, Biotechnology Medical University of Graz (Austria, 2018).
Result: The protein spots were isolated between 15-140 kDa ranges using 3-10 non-linear IPG strips and Brilliant Blue Coomassie. Ten proteins were characterized as immunogenic proteins, seven of them were identified and three of them were unknown proteins.
Conclusion: This study provided additional information about the somatic antigens of T. cati, which can lead to the development of new strategies for novel immuno-modulators, drug targets, subunit vaccines and immunodiagnostic kits for toxocariasis.
en
dc.language.iso
en
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dc.publisher
IRANIAN SCIENTIFIC SOCIETY MEDICAL ENTOMOLOGY
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dc.relation.ispartof
Iranian Journal of Parasitology
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dc.subject
Infectious Diseases
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dc.subject
Mass spectrometry
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dc.subject
Immunoblot
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dc.subject
Somatic extract
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dc.subject
Toxocariasis.
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dc.subject
Parasitology
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dc.title
Identification and Immunological Characterization of Somatic Proteins from Adults of Toxocara cati by Proteomics Technique
en
dc.type
Artikel
de
dc.type
Article
en
dc.type.category
Original Research Article
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tuw.journal.peerreviewed
true
-
tuw.peerreviewed
true
-
tuw.researchTopic.id
M6
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tuw.researchTopic.name
Biological and Bioactive Materials
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tuw.researchTopic.value
100
-
dcterms.isPartOf.title
Iranian Journal of Parasitology
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tuw.publication.orgunit
E164-01-3 - Forschungsgruppe Bioanalytik
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tuw.publisher.doi
10.18502/ijpa.v16i1.5508
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dc.identifier.eissn
2008-238X
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dc.description.numberOfPages
9
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wb.sci
true
-
wb.sciencebranch
Chemie
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wb.sciencebranch
Biologie
-
wb.sciencebranch.oefos
1040
-
wb.sciencebranch.oefos
1060
-
wb.facultyfocus
Bioscience Technology
de
wb.facultyfocus
Bioscience Technology
en
wb.facultyfocus.faculty
E150
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item.cerifentitytype
Publications
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item.openairecristype
http://purl.org/coar/resource_type/c_2df8fbb1
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item.grantfulltext
restricted
-
item.fulltext
no Fulltext
-
item.languageiso639-1
en
-
item.openairetype
research article
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crisitem.author.dept
E164-01 - Forschungsbereich Imaging und Instrumentelle Analytische Chemie
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crisitem.author.orcid
0000-0003-3950-0312
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crisitem.author.parentorg
E164 - Institut für Chemische Technologien und Analytik