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<div class="csl-entry">Akhgar, C. K., Ramos Garcia, V., Nürnberger, V., Moreno Giménez, A., Kuligowski, J., Rosenberg, E., Schwaighofer, A., & Lendl, B. (2022). Solvent-Free Lipid Separation and Attenuated Total Reflectance Infrared Spectroscopy for Fast and Green Fatty Acid Profiling of Human Milk. <i>Foods</i>, <i>11</i>(23), Article 3906. https://doi.org/10.3390/foods11233906</div>
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dc.identifier.issn
2304-8158
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/139245
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dc.description.abstract
This study presents the first mid-infrared (IR)-based method capable of simultaneously predicting concentrations of individual fatty acids (FAs) and relevant sum parameters in human milk (HM). Representative fat fractions of 50 HM samples were obtained by rapid, two-step centrifugation and subsequently measured with attenuated total reflection IR spectroscopy. Partial least squares models were compiled for the acquired IR spectra with gas chromatography-mass spectrometry (GC-MS) reference data. External validation showed good results particularly for the most important FA sum parameters and the following individual FAs: C12:0 (R²p = 0.96), C16:0 (R²p = 0.88), C18:1cis (R²p = 0.92), and C18:2cis (R²p = 0.92). Based on the obtained results, the effect of different clinical parameters on the HM FA profile was investigated, indicating a change of certain sum parameters over the course of lactation. Finally, assessment of the method’s greenness revealed clear superiority compared to GC-MS methods. The reported method thus represents a high-throughput, green alternative to resource-intensive established techniques.
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dc.description.sponsorship
European Commission
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dc.description.sponsorship
FFG - Österr. Forschungsförderungs- gesellschaft mbH
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dc.language.iso
en
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dc.publisher
MDPI
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dc.relation.ispartof
Foods
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dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
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dc.subject
mid-infrared spectroscopy
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dc.subject
attenuated total reflection
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dc.subject
human milk
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dc.subject
green analytical chemistry
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dc.subject
fatty acids
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dc.subject
partial least squares
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dc.title
Solvent-Free Lipid Separation and Attenuated Total Reflectance Infrared Spectroscopy for Fast and Green Fatty Acid Profiling of Human Milk