We introduce a new approach to reduce uncorrelated background signals from fluorescence imaging data, using real-time subtraction of background light. Our method allows removing image artifacts that cause signal clipping in a conventional imaging setup.
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Stimulated emission
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Stray ligh
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Lasers and electrooptics
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Fluorescence
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Adaptive optics
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Dynamic stray light and background correction to allow truly simultaneous optical stimulation and multiphoton imaging