Gundinger, T., Kittler, S., Kubicek, S., Kopp, J., & Spadiut, O. (2022). Recombinant Protein Production in E. coli Using the phoA Expression System. Fermentation, 8(4), Article 181. https://doi.org/10.3390/fermentation8040181
Auto‐inducible promoter systems have been reported to increase soluble product formation in the periplasm of E. coli compared to inducer‐dependent systems. In this study, we investigated the phosphate (PO₄)‐sensitive phoA expression system (pAT) for the production of a recombinant model antigen‐binding fragment (Fab) in the periplasm of E. coli in detail. We explored the impact of non‐limiting and limiting PO₄ conditions on strain physiology as well as Fab productivity. We compared different methods for extracellular PO₄ detection, identifying automated colorimetric measurement to be most suitable for at‐line PO₄ monitoring. We showed that PO₄ limitation boosts phoA‐based gene expression, however, the product was already formed at non‐limiting PO₄ conditions, indicating leaky expression. Furthermore, cultivation under PO₄ limitation caused physiological changes ultimately resulting in a metabolic breakdown at PO₄ starvation. Finally, we give recommendations for process optimization with the phoA expression system. In summary, our study provides very detailed information on the E. coli phoA expression system, thus extending the existing knowledge of this system, and underlines its high potential for the successful production of periplasmic products in E. coli.
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Research Areas:
Biological and Bioactive Materials: 50% Efficient Utilisation of Material Resources: 50%
