<div class="csl-bib-body">
<div class="csl-entry">Thyrhaug, E., Krause, S., Perri, A., Cerullo, G., Polli, D., Vosch, T., & Hauer, J. (2019). Single-molecule excitation-emission spectroscopy. <i>Proceedings of the National Academy of Sciences of the United States of America</i>, <i>116</i>(10), 4064–4069. https://doi.org/10.1073/pnas.1808290116</div>
</div>
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dc.identifier.issn
0027-8424
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/143699
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dc.description.abstract
Single-molecule spectroscopy (SMS) provides a detailed view of individual emitter properties and local environments without having to resort to ensemble averaging. While the last several decades have seen substantial refinement of SMS techniques, recording excitation spectra of single emitters still poses a significant challenge. Here we address this problem by demonstrating simultaneous collection of fluorescence emission and excitation spectra using a compact common-path interferometer and broadband excitation, which is implemented as an extension of a standard SMS microscope. We demonstrate the technique by simultaneously collecting room-temperature excitation and emission spectra of individual terrylene diimide molecules and donor–acceptor dyads embedded in polystyrene. We analyze the resulting spectral parameters in terms of optical lineshape theory to obtain detailed information on the interactions of the emitters with their nanoscopic environment. This analysis finally reveals that environmental fluctuations between the donor and acceptor in the dyads are not correlated.
en
dc.language.iso
en
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dc.publisher
National Academy of Sciences
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dc.relation.ispartof
Proceedings of the National Academy of Sciences of the United States of America
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dc.subject
Multidisciplinary
en
dc.title
Single-molecule excitation-emission spectroscopy
en
dc.type
Artikel
de
dc.type
Article
en
dc.description.startpage
4064
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dc.description.endpage
4069
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dc.type.category
Original Research Article
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tuw.container.volume
116
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tuw.container.issue
10
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tuw.journal.peerreviewed
true
-
tuw.peerreviewed
true
-
wb.publication.intCoWork
International Co-publication
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tuw.researchTopic.id
Q1
-
tuw.researchTopic.id
M2
-
tuw.researchTopic.id
I8
-
tuw.researchTopic.name
Photonics
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tuw.researchTopic.name
Materials Characterization
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tuw.researchTopic.name
Sensor Systems
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tuw.researchTopic.value
60
-
tuw.researchTopic.value
20
-
tuw.researchTopic.value
20
-
dcterms.isPartOf.title
Proceedings of the National Academy of Sciences of the United States of America
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tuw.publication.orgunit
E387-01 - Forschungsbereich Photonik
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tuw.publisher.doi
10.1073/pnas.1808290116
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dc.identifier.eissn
1091-6490
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dc.description.numberOfPages
6
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tuw.author.orcid
0000-0002-7062-8472
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tuw.author.orcid
0000-0002-1283-8535
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wb.sci
true
-
wb.sciencebranch
Elektrotechnik, Elektronik, Informationstechnik
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wb.sciencebranch.oefos
2020
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wb.facultyfocus
Photonik
de
wb.facultyfocus
Photonics
en
wb.facultyfocus.faculty
E350
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item.grantfulltext
none
-
item.cerifentitytype
Publications
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item.fulltext
no Fulltext
-
item.languageiso639-1
en
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item.openairecristype
http://purl.org/coar/resource_type/c_2df8fbb1
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item.openairetype
research article
-
crisitem.author.dept
E387 - Institut für Photonik
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crisitem.author.dept
E387 - Institut für Photonik
-
crisitem.author.parentorg
E350 - Fakultät für Elektrotechnik und Informationstechnik
-
crisitem.author.parentorg
E350 - Fakultät für Elektrotechnik und Informationstechnik