ADApt: Aspergillus-detecting aptamers

Abstract

The rapid technological, climatic and demographic changes characterizing the last few decades are exerting drastic effects on our planet. In this scenario, the occurrence of infectious diseases, including fungal infections, appears to increase steadily. Aspergillosis, caused by Aspergillus fungal species, poses a particular threat as it is estimated to affect millions of people every year. The high mortality rate of aspergillosis is often associated with delayed diagnosis. This project aims at the development and selection of DNA aptamers recognizing Aspergillus cells which can be implemented into a novel diagnostic tool for the detection of aspergillosis. Firstly, a previously developed SELEX approach [1] will be used to select DNA aptamers which specifically recognize conidia and hyphae of Aspergillus species causing aspergillosis. Secondly, developed aptamers will be characterized for their binding affinity and specificity by establishing an evaluation method based on flow cytometry and cell sorting. Aspergillus-specific aptamers developed in this project will be further developed into novel diagnostic tools to detect aspergillosis. ____ [1] Kolm C et al. Sci Rep. 2020;10:1–16.