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<div class="csl-entry">Kittler, S., Ebner, J., Besleaga, M., Larsbrink, J., Darnhofer, B., Birner-Grünberger, R., Schobesberger, S., Akhgar, C. K., Schwaighofer, A., Lendl, B., & Spadiut, O. (2022). Recombinant Protein L: Production, Purification and Characterization of a Universal Binding Ligand. <i>Journal of Biotechnology</i>, <i>359</i>, 108–115. https://doi.org/10.1016/j.jbiotec.2022.10.002</div>
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dc.identifier.issn
0168-1656
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/144328
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dc.description.abstract
Protein L (PpL) is a universal binding ligand that can be used for the detection and purification of antibodies and antibody fragments. Due to the unique interaction with immunoglobulin light chains, it differs from other affinity ligands, like protein A or G. However, due to its current higher market price, PpL is still scarce in applications. In this study, we investigated the recombinant production and purification of PpL and characterized the product in detail. We present a comprehensive roadmap for the production of the versatile protein PpL in E. coli.
en
dc.language.iso
en
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dc.publisher
ELSEVIER
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dc.relation.ispartof
Journal of Biotechnology
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dc.subject
Protein L
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dc.subject
E. coli
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dc.subject
Recombinant production
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dc.subject
Bioreactor
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dc.subject
Affinity ligand
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dc.title
Recombinant Protein L: Production, Purification and Characterization of a Universal Binding Ligand