<div class="csl-bib-body">
<div class="csl-entry">Brameshuber, M., Kellner, F., Rossboth, B., Ta, H., Alge, K., Sevcsik, E., Göhring, J., Axmann, M., Baumgart, F., Gascoigne, N. R. J., Darvis, S. J., Stockinger, H., Schütz, G., & Huppa, J. B. (2018). Monomeric TCRs Drive T Cell Antigen Recognition. <i>Nature Immunology</i>, <i>19</i>(5), 487–496. https://doi.org/10.1038/s41590-018-0092-4</div>
</div>
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dc.identifier.issn
1529-2908
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/144517
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dc.description.abstract
T cell antigen recognition requires T cell antigen receptors (TCRs) engaging MHC-embedded antigenic peptides (pMHCs)
within the contact region of a T cell with its conjugated antigen-presenting cell. Despite micromolar TCR:pMHC affinities,
T cells respond to even a single antigenic pMHC, and higher-order TCRs have been postulated to maintain high antigen sensitivity
and trigger signaling. We interrogated the stoichiometry of TCRs and their associated CD3 subunits on the surface of living
T cells through single-molecule brightness and single-molecule coincidence analysis, photon-antibunching-based fluorescence
correlation spectroscopy and Förster resonance energy transfer measurements. We found exclusively monomeric TCR-
CD3 complexes driving the recognition of antigenic pMHCs, which underscores the exceptional capacity of single TCR-CD3
complexes to elicit robust intracellular signaling.
