<div class="csl-bib-body">
<div class="csl-entry">Kiesenhofer, D. P., Mach, R. L., & Mach-Aigner, A. R. (2018). Influence of cis Element Arrangement on Promoter Strength in Trichoderma reesei. <i>Applied and Environmental Microbiology</i>, <i>84</i>(1). https://doi.org/10.1128/aem.01742-17</div>
</div>
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dc.identifier.issn
0099-2240
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/144820
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dc.description.abstract
Trichoderma reesei can produce up to 100 g/liter of extracellular proteins. The major and industrially relevant products are cellobiohydrolase I (CBHI) and the hemicellulase XYNI. The genes encoding both enzymes are transcriptionally activated by the regulatory protein Xyr1. The first 850 nucleotides of the cbh1 promoter contain 14 Xyr1-binding sites (XBS), and 8 XBS are present in the xyn1 promoter. Some of these XBS are arranged in tandem and others as inverted repeats. One such cis element, an inverted repeat, plays a crucial role in the inducibility of the xyn1 promoter. We investigated the impact of the properties of such cis elements by shuffling them by insertion, exchange, deletion, and rearrangement of cis elements in both the cbh1 and xyn1 promoter. A promoter-reporter assay using the Aspergillus niger goxA gene allowed us to measure changes in the promoter strength and inducibility. Most strikingly, we found that an inverted repeat of XBS causes an important increase in cbh1 promoter strength and allows induction by xylan or wheat straw. Furthermore, evidence is provided that the distances of cis elements to the transcription start site have important influence on promoter activity. Our results suggest that the arrangement and distances of cis elements have large impacts on the strength of the cbh1 promoter, whereas the sheer number of XBS has only secondary importance. Ultimately, the biotechnologically important cbh1 promoter can be improved by cis element rearrangement.
IMPORTANCE In the present study, we demonstrate that the arrangement of cis elements has a major impact on promoter strength and inducibility. We discovered an influence on promoter activity by the distances of cis elements to the transcription start site. Furthermore, we found that the configuration of cis elements has a greater effect on promoter strength than does the sheer number of transactivator binding sites present in the promoter. Altogether, the arrangement of cis elements is an important factor that should not be overlooked when enhancement of gene expression is desired.
en
dc.language.iso
en
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dc.publisher
AMER SOC MICROBIOLOGY
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dc.relation.ispartof
Applied and Environmental Microbiology
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dc.subject
Biotechnology
en
dc.subject
Food Science
en
dc.subject
Applied Microbiology and Biotechnology
en
dc.subject
Ecology
en
dc.title
Influence of cis Element Arrangement on Promoter Strength in Trichoderma reesei
en
dc.type
Artikel
de
dc.type
Article
en
dc.type.category
Original Research Article
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tuw.container.volume
84
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tuw.container.issue
1
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tuw.journal.peerreviewed
true
-
tuw.peerreviewed
true
-
tuw.researchTopic.id
E6
-
tuw.researchTopic.id
E5
-
tuw.researchTopic.name
Sustainable Production and Technologies
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tuw.researchTopic.name
Efficient Utilisation of Material Resources
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tuw.researchTopic.value
50
-
tuw.researchTopic.value
50
-
dcterms.isPartOf.title
Applied and Environmental Microbiology
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tuw.publication.orgunit
E166-05-1 - Forschungsgruppe Synthetische Biologie und Molekulare Biotechnologie
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tuw.publisher.doi
10.1128/aem.01742-17
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dc.identifier.eissn
1098-5336
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dc.description.numberOfPages
15
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wb.sci
true
-
wb.sciencebranch
Chemische Verfahrenstechnik
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wb.sciencebranch.oefos
2040
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wb.facultyfocus
Bioscience Technology
de
wb.facultyfocus
Bioscience Technology
en
wb.facultyfocus.faculty
E150
-
item.languageiso639-1
en
-
item.openairecristype
http://purl.org/coar/resource_type/c_2df8fbb1
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item.openairetype
research article
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item.grantfulltext
none
-
item.fulltext
no Fulltext
-
item.cerifentitytype
Publications
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crisitem.author.dept
E166 - Institut für Verfahrenstechnik, Umwelttechnik und technische Biowissenschaften
-
crisitem.author.dept
E166 - Institut für Verfahrenstechnik, Umwelttechnik und technische Biowissenschaften
-
crisitem.author.dept
E166-05 - Forschungsbereich Biochemische Technologie
-
crisitem.author.orcid
0000-0003-2375-7244
-
crisitem.author.orcid
0000-0002-7575-2317
-
crisitem.author.parentorg
E150 - Fakultät für Technische Chemie
-
crisitem.author.parentorg
E150 - Fakultät für Technische Chemie
-
crisitem.author.parentorg
E166 - Institut für Verfahrenstechnik, Umwelttechnik und technische Biowissenschaften