<div class="csl-bib-body">
<div class="csl-entry">Zoratto, S., Avdic, S., Kremslehner, C., Sochorová, M., Gruber Florian, & Marchetti-Deschmann, M. (2022, July 6). <i>A systematic study of lipids – from ESI analysis to MALDI Imaging using a 7T FT-ICR instrument</i> [Conference Presentation]. 32nd Mass Spec Forum, Vienna, Austria. http://hdl.handle.net/20.500.12708/148082</div>
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/148082
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dc.description.abstract
Lipids are essential molecules for cellular signaling, metabolism, and key components of our cells. At the skin level, exposure to solar radiation, high oxygen levels, and environmental pollutants leads to premature skin aging, tissue inflammation, and photocarcinogenesis. This oxidative stress activates cutaneous lipoxygenases or causes nonenzymatic lipid peroxidation, thus forming oxidized lipids (oxLipS). These oxLipS are crucial components in events like cellular senescence. We present a systematic investigation of lipids and their non-enzymtic oxidation products generated in a controlled environment by reactive oxygen species (ROS) after UV exposure. Untargeted OxLipS analysis was performed on a dual-source ESI/MALDI 7T scimaX FTICR MRMS (Bruker). ESI provides high throughput and high sensitivity, while the MALDI Imaging platform (MSI) complements with the spatial localization.
A lipid standard solution (LipS), containing 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine (PAPC) and not UV affected references 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-dinonanoyl-sn-glycero-3-phosphocholine (DNPC) in chloroform were exposed to solar-like conditions (UVA radiation). Direct infusion ESI experiments of the LipS generated crucial information on oxLipS species produced by ROS formed in solution. Accurate, high-resolution MS data allowed identification of known and newly formed species via database search (LipidMAPS). A tentative structural assignment was performed for m/z values not found in the database.
Skin biopsies were mounted on ITO slides for MALDI MSI. Different sample preparation methods (sublimation, spraying) after tissue washing have been investigated, and instrument parameters adapted for optimized FT ICR imaging [1,2]. Selected m/z values were compared between ESI and MALDI, thus aiding the translation of ex vivo experiments to in vivo MALDI MSI results. Here, several oxLipS were localized, visualized, and identified in skin section samples. The different sample preparation approaches allowed us to further improve and develop a method for oxLipS investigation in skin tissue section via MALDI MSI.
en
dc.description.sponsorship
CDG Christian Doppler Forschungsgesellschaft
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dc.language.iso
en
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dc.subject
FT MS, Mass Spectrometry
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dc.title
A systematic study of lipids – from ESI analysis to MALDI Imaging using a 7T FT-ICR instrument
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dc.type
Presentation
en
dc.type
Vortrag
de
dc.contributor.affiliation
Technische Universität Wien, Austria
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dc.contributor.affiliation
Technische Universität Wien, Austria
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dc.contributor.affiliation
Christian Doppler Laboratory on Biotechnology of Skin Aging, Vienna, Austria
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dc.contributor.affiliation
Medical University of Vienna, Austria
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dc.contributor.affiliation
University of Vienna, Austria
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dc.contributor.affiliation
Technische Universität Wien, Austria
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dc.relation.grantno
SKINMAGINE
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dc.type.category
Conference Presentation
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tuw.project.title
CDL für Multimodales Analytisches Imaging von Seneszenz und Alterung der Haut
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tuw.researchTopic.id
M6
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tuw.researchTopic.name
Biological and Bioactive Materials
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tuw.researchTopic.value
100
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tuw.publication.orgunit
E164-01-1 - Forschungsgruppe Massenspektrometrische Bio- und Polymeranalytik
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tuw.author.orcid
0000-0002-1491-3404
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tuw.author.orcid
0000-0001-9746-196X
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tuw.author.orcid
0000-0003-0273-3702
-
tuw.author.orcid
0000-0003-1094-5641
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tuw.author.orcid
0000-0002-8060-7851
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tuw.event.name
32nd Mass Spec Forum
en
tuw.event.startdate
05-07-2022
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tuw.event.enddate
06-07-2022
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tuw.event.online
On Site
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tuw.event.type
Event for scientific audience
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tuw.event.place
Vienna
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tuw.event.country
AT
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tuw.event.institution
University of Vienna and TU Wien
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tuw.event.presenter
Zoratto, Samuele
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tuw.event.track
Single Track
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wb.sciencebranch
Chemie
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wb.sciencebranch.oefos
1040
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wb.sciencebranch.value
100
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item.cerifentitytype
Publications
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item.fulltext
no Fulltext
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item.languageiso639-1
en
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item.openairetype
conference paper not in proceedings
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item.openairecristype
http://purl.org/coar/resource_type/c_18cp
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item.grantfulltext
none
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crisitem.author.dept
E164-01-1 - Forschungsgruppe Massenspektrometrische Bio- und Polymeranalytik
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crisitem.author.dept
Christian Doppler Laboratory on Biotechnology of Skin Aging, Vienna, Austria
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crisitem.author.dept
Medical University of Vienna
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crisitem.author.dept
University of Vienna
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crisitem.author.dept
E164 - Institut für Chemische Technologien und Analytik
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crisitem.author.orcid
0000-0002-1491-3404
-
crisitem.author.orcid
0000-0001-9746-196X
-
crisitem.author.orcid
0000-0003-0273-3702
-
crisitem.author.orcid
0000-0002-8060-7851
-
crisitem.author.parentorg
E164-01 - Forschungsbereich Imaging und Instrumentelle Analytische Chemie
