<div class="csl-bib-body">
<div class="csl-entry">Schierscher, T. (2020). <i>Stress-protective genes from tardigrades</i> [Diploma Thesis, Technische Universität Wien]. reposiTUm. https://doi.org/10.34726/hss.2020.66501</div>
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dc.identifier.uri
https://doi.org/10.34726/hss.2020.66501
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/15150
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dc.description
Abweichender Titel nach Übersetzung der Verfasserin/des Verfassers
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dc.description.abstract
From obtained stress-resistant yeast clones expressing tardigrade genes, plasmids are isolated, trans-formed into E. coli and then back-transformed into naive yeast (S. cerevisiae). Plasmids conferring stress tolerance are sequenced and sequence homologs from other species are identified through BLAST search on the amino acid level. Patterns of cross-resistance to multiple stressors (heat stress, oxidative stress, hyperosmosis) are examined among the transformants using the Scan-O-Matic microbial colony growth screening press and by recording growth in liquid media using Bioscreen. It was shown that a tardigrade gene that codes for a serum paraoxonase/arylesterase 1 is enhancing the resistance of S. cerevisiae to heat stress and osmotic stress.
en
dc.language
English
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dc.language.iso
en
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dc.rights.uri
http://rightsstatements.org/vocab/InC/1.0/
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dc.subject
Bärtierchen
de
dc.subject
Stressgene
de
dc.subject
Tardigrades
en
dc.subject
stress genes
en
dc.title
Stress-protective genes from tardigrades
en
dc.title.alternative
Stressschützende Gene von Tardigrada
de
dc.type
Thesis
en
dc.type
Hochschulschrift
de
dc.rights.license
In Copyright
en
dc.rights.license
Urheberrechtsschutz
de
dc.identifier.doi
10.34726/hss.2020.66501
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dc.contributor.affiliation
TU Wien, Österreich
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dc.rights.holder
Tobias Schierscher
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dc.publisher.place
Wien
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tuw.version
vor
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tuw.thesisinformation
Technische Universität Wien
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tuw.publication.orgunit
E166 - Institut für Verfahrenstechnik, Umwelttechnik und technische Biowissenschaften