<div class="csl-bib-body">
<div class="csl-entry">Pekarsky, A., & Spadiut, O. (2022). Dynamic Feeding for Pichia pastoris. In V. Mapelli & M. Bettiga (Eds.), <i>Yeast Metabolic Engineering</i> (Vol. 2513, pp. 243–254). https://doi.org/10.1007/978-1-0716-2399-2_14</div>
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/152248
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dc.description.abstract
The knowledge of certain strain-specific parameters of recombinant Pichia pastoris strains is required to be able to set up a feeding regime for fed-batch cultivations. These parameters are commonly determined either by time-consuming and labor-intensive continuous cultivations or by several, consecutive fed-batch cultivations. Here, we describe a fast method based on batch experiments with substrate pulses to extract certain strain characteristic parameters, which are required to set up a dynamic feeding strategy for P. pastoris strains based on the specific substrate uptake rate. We further describe in detail the course of actions, which have to be taken to obtain the desired dynamics during feeding.
en
dc.language.iso
en
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dc.subject
Recombinant Proteins
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dc.subject
Dynamic fed-batch strategy
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dc.subject
Pichia pastoris
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dc.subject
Specific substrate uptake rate
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dc.subject
Strain characterization
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dc.subject
Strain physiology
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dc.subject
Substrate pulses
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dc.subject
Yeast
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dc.subject
Pichia
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dc.subject
Saccharomycetales
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dc.title
Dynamic Feeding for Pichia pastoris
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dc.type
Book Contribution
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dc.type
Buchbeitrag
de
dc.contributor.editoraffiliation
Chalmers University of Technology in Gothenburg, Sweden
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dc.contributor.editoraffiliation
Chalmers University of Technology in Gothenburg, Sweden