<div class="csl-bib-body">
<div class="csl-entry">Masi, A. L., Stark, G., Pfnier, J., Daza Serna, L. V., Mach-Aigner, A., & Mach, R. (2022, September 22). <i>Erythritol production by the saprobe Trichoderma reesei: screening and optimization using Design of Experiments to better understand the effect of nitrogen source, carbon source, pH and temperature.</i> [Conference Presentation]. 14th ÖGMBT Annual Meeting, Vienna, Austria.</div>
</div>
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/152445
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dc.description
Short talk (12 min presentation and 3 min questions) in the session "Synthetic Microbiology for Bioprocesses"
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dc.description.abstract
Erythritol is a four-carbon polyol, widely occurring in nature in mushrooms and fruits. It is also found in fermented food and
drinks such as soy sauce, wine, or beer due to its production by fungi and some lactic acid bacteria. Its specific properties
make it a sweetener of growing interest. Erythritol is reported to have minor side effects on the digestive tracks. Additionally,
no effect on sugar or insulin level has been reported, nor has it any impact on the gut microbiota, making erythritol one of
the safest sweeteners reported so far. But despite all its advantages, erythritol use is still limited in the food industry due to
its high manufacturing cost.
The high cost of erythritol is partially due to the use of glucose as a substrate. At the industrial scale, the leading producers
of erythritol are osmophilic yeasts, mainly Moniliella pollinis. They use glucose, obtained by hydrolyzing corn or potato
starch, and metabolize it into erythritol. Strategies to improve erythritol manufacturing have been oriented around four
axes: improve producer strains by genetic engineering, develop the use of cheaper substrates, better understand polyols
metabolism and optimize the manufacturing process.
Due to the European regulation on GMOs, interest has been growing in producing erythritol from non-GMO strains. If strains
cannot be engineered, the main remaining axes for improvement are understanding erythritol production better, improving
and optimizing the production process, and finding cheaper substrates. In this logic, we worked on developing a production
process with Trichoderma reesei, a saprobe, using agro-industrial waste as substrates. The presented work focused on using
Design of Experiments (DoE) to screen the effect of 4 process parameters: nitrogen source, carbon source, pH, and
temperature. An optimum in the tested range was also proposed. This work also brought hypotheses on how these
parameters modify polyol production
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dc.language.iso
en
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dc.subject
Erythritol, polyols, Trichoderma, design of experiments
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dc.title
Erythritol production by the saprobe Trichoderma reesei: screening and optimization using Design of Experiments to better understand the effect of nitrogen source, carbon source, pH and temperature.
en
dc.type
Presentation
en
dc.type
Vortrag
de
dc.rights.holder
ÖGMBT
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dc.type.category
Conference Presentation
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tuw.researchTopic.id
E6
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tuw.researchTopic.id
X1
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tuw.researchTopic.name
Sustainable Production and Technologies
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tuw.researchTopic.name
Beyond TUW-research foci
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tuw.researchTopic.value
50
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tuw.researchTopic.value
50
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tuw.publication.orgunit
E166-05-1 - Forschungsgruppe Synthetische Biologie und Molekulare Biotechnologie
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tuw.publication.orgunit
E166 - Institut für Verfahrenstechnik, Umwelttechnik und technische Biowissenschaften
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tuw.author.orcid
0000-0001-5852-1528
-
tuw.author.orcid
0000-0002-7575-2317
-
tuw.author.orcid
0000-0003-2375-7244
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tuw.event.name
14th ÖGMBT Annual Meeting
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tuw.event.startdate
19-09-2022
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tuw.event.enddate
22-09-2022
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tuw.event.online
On Site
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tuw.event.type
Event for scientific audience
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tuw.event.place
Vienna
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tuw.event.country
AT
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tuw.event.institution
ÖGMBT
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tuw.event.presenter
Masi, Audrey Laura
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tuw.event.track
Multi Track
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wb.sciencebranch
Chemische Verfahrenstechnik
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wb.sciencebranch
Biologie
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wb.sciencebranch
Industrielle Biotechnologie
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wb.sciencebranch.oefos
2040
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wb.sciencebranch.oefos
1060
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wb.sciencebranch.oefos
2090
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wb.sciencebranch.value
10
-
wb.sciencebranch.value
50
-
wb.sciencebranch.value
40
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item.openairetype
Presentation
-
item.openairetype
Vortrag
-
item.grantfulltext
none
-
item.cerifentitytype
Publications
-
item.cerifentitytype
Publications
-
item.languageiso639-1
en
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item.openairecristype
http://purl.org/coar/resource_type/c_18cf
-
item.openairecristype
http://purl.org/coar/resource_type/c_18cf
-
item.fulltext
no Fulltext
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crisitem.author.dept
E166-05-1 - Forschungsgruppe Synthetische Biologie und Molekulare Biotechnologie
-
crisitem.author.dept
E166-05-1 - Forschungsgruppe Synthetische Biologie und Molekulare Biotechnologie
-
crisitem.author.dept
E166-05-1 - Forschungsgruppe Synthetische Biologie und Molekulare Biotechnologie
-
crisitem.author.dept
E166-05-1 - Forschungsgruppe Synthetische Biologie und Molekulare Biotechnologie
-
crisitem.author.dept
E166-05-1 - Forschungsgruppe Synthetische Biologie und Molekulare Biotechnologie
-
crisitem.author.dept
E166 - Institut für Verfahrenstechnik, Umwelttechnik und technische Biowissenschaften
-
crisitem.author.orcid
0000-0001-5852-1528
-
crisitem.author.orcid
0000-0002-7575-2317
-
crisitem.author.orcid
0000-0003-2375-7244
-
crisitem.author.parentorg
E166-05 - Forschungsbereich Biochemische Technologie
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crisitem.author.parentorg
E166-05 - Forschungsbereich Biochemische Technologie
-
crisitem.author.parentorg
E166-05 - Forschungsbereich Biochemische Technologie
-
crisitem.author.parentorg
E166-05 - Forschungsbereich Biochemische Technologie
-
crisitem.author.parentorg
E166-05 - Forschungsbereich Biochemische Technologie