<div class="csl-bib-body">
<div class="csl-entry">Zoratto, S., Kremslehner, C., Sochorová, M., Gruber, F., & Marchetti-Deschmann, M. (2022, July 13). <i>From ESI Analysis To MALDI Imaging – Studying Lipid Oxidation on a 7T Maldi FT-ICR Instrument</i> [Conference Presentation]. EFTMS 2022, Lisbon, Portugal. http://hdl.handle.net/20.500.12708/152494</div>
</div>
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/152494
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dc.description.abstract
Lipids are essential molecules for cellular signaling, metabolism, and key components of our cells. At the skin level, exposure to solar radiation, high oxygen levels, and environmental pollutants leads to premature skin aging, tissue inflammation, and photocarcinogenesis. This oxidative stress activates cutaneous lipoxygenases or causes nonenzymatic lipid peroxidation, thus forming oxidized lipids (oxLipS). We present a systematic investigation of lipids and their non-enzymtic oxidation products generated in a controlled environment after UV exposure. Untargeted OxLipS analysis was performed on a dual-source ESI/MALDI 7T scimaX FTICR MRMS (Bruker). ESI was used for species identification, while the MALDI Imaging platform (MSI) complemented the analysis with spatial localization in tissue.
A lipid standard solution (LipS), PAPC and not UV affected references DPPC and DNPC in chloroform were exposed to solar-like conditions (UVA radiation). Direct infusion ESI experiments of the LipS generated crucial information on oxLipS species produced by ROS formed in solution. Accurate, high-resolution MS data allowed identification of known and newly formed species via database search (LipidMAPS).
Skin biopsies were mounted on ITO slides for MALDI MSI. Different sample preparation methods after tissue washing have been investigated, and instrument parameters adapted for optimized FT ICR imaging [1,2]. Selected m/z values were compared between ESI and MALDI, thus aiding the translation of ex vivo experiments to in vivo MALDI MSI results. Several oxLipS were localized, visualized, and identified in skin section samples.
REFERENCES
[1] Narzt MS et al. (2021) J Invest Dermatol. 141(4S):993-1006.e15. doi: 10.1016/j.jid.2020.11.020
[2] Tiquet et al. (2022) Anal. Chem. , doi.org/10.1021/acs.analchem.2c00754
en
dc.description.sponsorship
CDG Christian Doppler Forschungsgesellschaft
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dc.language.iso
en
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dc.subject
FT ICR, Mass Spectrometry
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dc.title
From ESI Analysis To MALDI Imaging – Studying Lipid Oxidation on a 7T Maldi FT-ICR Instrument
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dc.type
Presentation
en
dc.type
Vortrag
de
dc.contributor.affiliation
Medical University of Vienna, Austria
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dc.relation.grantno
SKINMAGINE
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dc.type.category
Conference Presentation
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tuw.project.title
CDL für Multimodales Analytisches Imaging von Seneszenz und Alterung der Haut
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tuw.researchTopic.id
M6
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tuw.researchTopic.name
Biological and Bioactive Materials
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tuw.researchTopic.value
100
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tuw.publication.orgunit
E164-01-1 - Forschungsgruppe Massenspektrometrische Bio- und Polymeranalytik
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tuw.author.orcid
0000-0002-1491-3404
-
tuw.author.orcid
0000-0001-9746-196X
-
tuw.author.orcid
0000-0003-0273-3702
-
tuw.author.orcid
0000-0003-1094-5641
-
tuw.author.orcid
0000-0002-8060-7851
-
tuw.event.name
EFTMS 2022
en
tuw.event.startdate
11-07-2022
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tuw.event.enddate
14-07-2022
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tuw.event.online
On Site
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tuw.event.type
Event for scientific audience
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tuw.event.place
Lisbon
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tuw.event.country
PT
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tuw.event.presenter
Marchetti-Deschmann, Martina
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wb.sciencebranch
Chemie
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wb.sciencebranch.oefos
1040
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wb.sciencebranch.value
100
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item.openairetype
conference paper not in proceedings
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item.languageiso639-1
en
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item.cerifentitytype
Publications
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item.fulltext
no Fulltext
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item.grantfulltext
none
-
item.openairecristype
http://purl.org/coar/resource_type/c_18cp
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crisitem.project.funder
Christian Doppler Forschungsgesells
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crisitem.project.grantno
CDL SKINMAGINE
-
crisitem.author.dept
E164-01-1 - Forschungsgruppe Massenspektrometrische Bio- und Polymeranalytik
-
crisitem.author.dept
Christian Doppler Research Association
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crisitem.author.dept
Medical University of Vienna
-
crisitem.author.dept
Medical University of Vienna
-
crisitem.author.dept
E164 - Institut für Chemische Technologien und Analytik
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crisitem.author.orcid
0000-0002-1491-3404
-
crisitem.author.orcid
0000-0001-9746-196X
-
crisitem.author.orcid
0000-0003-0273-3702
-
crisitem.author.orcid
0000-0002-8060-7851
-
crisitem.author.parentorg
E164-01 - Forschungsbereich Imaging und Instrumentelle Analytische Chemie
