<div class="csl-bib-body">
<div class="csl-entry">Schwaighofer, A., Akhgar, C. K., Ebner, J., Alcaraz, M. R., Kopp, J., Goicoechea, H., Spadiut, O., & Lendl, B. (2022, June 23). <i>Hyphenation of Preparative Liquid Chromatography to Laser-Based Mid-IR Spectroscopy for Monitoring of Proteins in Chromatographic Effluents</i> [Poster Presentation]. Analytica 2022, München, Germany.</div>
</div>
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/152536
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dc.description.abstract
Mid-infrared spectroscopy provides detailed information about proteins that cannot be obtained with conventional UV detectors. The most powerful IR bands for protein quantification and secondary structure analysis are the amide I (1700-1600 cm-1) and amide II (1600-1500 cm-1) bands.
A pronounced challenge in analysis of aqueous protein solutions is the strong absorption of H2O at approx. 1640 cm-1 that overlaps with the amide I band. In conventional Fourier-transform infrared (FTIR) spectroscopy, the optical path-length is restricted to <10 µm to avoid total IR absorption. These small path lengths are unsuitable for flow-through operations due to low robustness.
External cavity-quantum cascade lasers (EC-QCLs) offer higher optical powers compared to the thermal sources used in FTIR spectroscopy, leading to increased sensitivity and larger applicable optical path lengths. These advantages open a wide range of possible applications, including near real-time protein monitoring from downstream operations.
Here, an EC-QCL based mid-IR spectrometer was coupled to a preparative liquid chromatography (LC) system. Two different model systems, based on ion-exchange chromatography (IEX) and size exclusion chromatography (SEC) were employed to demonstrate the high flexibility of LC-QCL-IR coupling.
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dc.description.sponsorship
FFG - Österr. Forschungsförderungs- gesellschaft mbH
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dc.description.sponsorship
Fonds zur Förderung der wissenschaftlichen Forschung (FWF)
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dc.language.iso
en
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dc.subject
mid-IR spectroscopy, protein separation, Quantum cascade laser, hyphenation
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dc.title
Hyphenation of Preparative Liquid Chromatography to Laser-Based Mid-IR Spectroscopy for Monitoring of Proteins in Chromatographic Effluents
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dc.type
Presentation
en
dc.type
Vortrag
de
dc.contributor.affiliation
Ciudad Universitaria, Santa Fe, Argentina
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dc.relation.grantno
868615
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dc.relation.grantno
P 32644-N
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dc.type.category
Poster Presentation
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tuw.project.title
Chemical Systems Engineering
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tuw.project.title
Advancing QCL-IR spectroscopy of proteins for DSP monitoring
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tuw.researchTopic.id
Q1
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tuw.researchTopic.id
M6
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tuw.researchTopic.name
Photonics
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tuw.researchTopic.name
Biological and Bioactive Materials
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tuw.researchTopic.value
50
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tuw.researchTopic.value
50
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tuw.publication.orgunit
E164-02-1 - Forschungsgruppe Prozessanalytik
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tuw.author.orcid
0000-0003-2714-7056
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tuw.author.orcid
0000-0003-3838-5842
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tuw.event.name
Analytica 2022
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tuw.event.startdate
21-06-2022
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tuw.event.enddate
24-06-2022
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tuw.event.online
On Site
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tuw.event.type
Event for scientific audience
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tuw.event.place
München
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tuw.event.country
DE
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tuw.event.presenter
Schwaighofer, Andreas
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wb.sciencebranch
Chemie
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wb.sciencebranch.oefos
1040
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wb.sciencebranch.value
100
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item.openairetype
Presentation
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item.openairetype
Vortrag
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item.grantfulltext
none
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item.cerifentitytype
Publications
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item.cerifentitytype
Publications
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item.languageiso639-1
en
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item.openairecristype
http://purl.org/coar/resource_type/c_18cf
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item.openairecristype
http://purl.org/coar/resource_type/c_18cf
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item.fulltext
no Fulltext
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crisitem.project.funder
FFG - Österr. Forschungsförderungs- gesellschaft mbH
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crisitem.project.funder
FWF Fonds zur Förderung der wissenschaftlichen Forschung (FWF)