<div class="csl-bib-body">
<div class="csl-entry">Zanetti, C., Föttinger-Vacha Alexandra, Stephan Christopher, & Marchetti-Deschmann, M. (2022, August 28). <i>MALDI-intact cell mass spectrometry as a monitoring tool for fermentation processes</i> [Conference Presentation]. JAF Young Analytical Chemists Forum, IFA Tulln, Austria. http://hdl.handle.net/20.500.12708/152892</div>
</div>
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/152892
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dc.description.abstract
MALDI-Intact Cell Mass Spectrometry (MALDI-ICMS) is a helpful technique for discriminating/identifying microorganisms (MALDI Biotyping); it is used in clinics for rapid pathogen identification in case of hospital-acquired infections (e.g., Staphylococcus aureus), enabling quick response and intervention. Industrial fermentations exploit microorganisms for synthetizing products of interest; while the failure of such fermentations is cost-intensive, process characterization can help decision-making for process optimization. Aiming at developing novel process monitoring tools, we present the use of MALDI-ICMS to monitor
E. coli fermentations.
Samples from E. coli bioreactors were collected at different fermentation times, aliquots were washed by pelletation and resuspension in a NH4HCO3 buffer (pH 7) and equalized to the same OD550, the cell suspensions were mixed with a MALDI matrix at a ratio of 1:2 (sinapinic acid/ferulic acid, 0.5 % trifluororacetic acid, 70 % acetonitrile). The mixture was spotted in replicates on a MALDI target and air dried. The samples were analyzed on a MALDI-TOF device (UltrafleXtreme, Bruker) in the positive linear mode (2-20 kDa mass range). By using multivariate data analysis in the R environment, we exploited time-dependent spectral changes to build a model capable of monitoring the fermentation progress. While principal component analysis could separate fermentation phases, partial least square regression could track fermentation progress with a prediction error of less than 5 % of the total fermentation time. The presented approach will allow better early-stage process characterization and prediction of process outcome.
en
dc.description.sponsorship
Boehringer Ingelheim RCV GmbH & Co KG
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dc.language.iso
en
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dc.subject
PAT, MALDI MS, Intact Cell Mass Spectrometry
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dc.title
MALDI-intact cell mass spectrometry as a monitoring tool for fermentation processes
en
dc.type
Presentation
en
dc.type
Vortrag
de
dc.contributor.affiliation
Boehringer Ingelheim (Germany), Germany
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dc.contributor.affiliation
Boehringer Ingelheim (Germany), Germany
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dc.type.category
Conference Presentation
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tuw.project.title
MALDI TOF MS biotyping
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tuw.researchTopic.id
M6
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tuw.researchTopic.name
Biological and Bioactive Materials
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tuw.researchTopic.value
100
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tuw.publication.orgunit
E164-01-1 - Forschungsgruppe Massenspektrometrische Bio- und Polymeranalytik
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tuw.author.orcid
0000-0001-5019-2408
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tuw.author.orcid
0000-0002-8060-7851
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tuw.event.name
JAF Young Analytical Chemists Forum
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tuw.event.startdate
28-08-2022
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tuw.event.enddate
28-08-2022
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tuw.event.online
On Site
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tuw.event.type
Event for scientific audience
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tuw.event.place
IFA Tulln
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tuw.event.country
AT
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tuw.event.institution
BOKU, ASAC
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tuw.event.presenter
Zanetti, Cristian
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tuw.event.track
Single Track
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wb.sciencebranch
Chemie
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wb.sciencebranch.oefos
1040
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wb.sciencebranch.value
100
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item.grantfulltext
none
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item.cerifentitytype
Publications
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item.fulltext
no Fulltext
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item.openairetype
conference paper not in proceedings
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item.openairecristype
http://purl.org/coar/resource_type/c_18cp
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item.languageiso639-1
en
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crisitem.author.dept
E164-01-1 - Forschungsgruppe Massenspektrometrische Bio- und Polymeranalytik
-
crisitem.author.dept
Boehringer Ingelheim (Germany)
-
crisitem.author.dept
Boehringer Ingelheim (Germany)
-
crisitem.author.dept
E164 - Institut für Chemische Technologien und Analytik
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crisitem.author.orcid
0000-0001-5019-2408
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crisitem.author.orcid
0000-0002-8060-7851
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crisitem.author.parentorg
E164-01 - Forschungsbereich Imaging und Instrumentelle Analytische Chemie
