Vierheilig, Julia

Dielacher, Irina

Slipko, Katarzyna Anna

Masseron, Amandine

Wögerbauer, Markus

Galazka, Sonia

Radu, Lacramioara-Elena

Derx, Julia

Linke, Rita Barbara

Cervero-Arago, Silvia

Krampe, Jörg

Kreuzinger, Norbert

2023-02-21T07:29:56Z

2023-02-21T07:29:56Z

2022-09-25

<div class="csl-bib-body"> <div class="csl-entry">Vierheilig, J., Dielacher, I., Slipko, K. A., Masseron, A., Wögerbauer, M., Galazka, S., Radu, L.-E., Derx, J., Linke, R. B., Cervero-Arago, S., Krampe, J., &#38; Kreuzinger, N. (2022, September 25). <i>Systematic Determination Of Antibiotic Resistance Genes In Soil, Wastewater And Surface Water In Austria</i> [Poster Presentation]. 6th International Symposium on the environmental dimension of antibiotic resistance, Gothenburg, Sweden. http://hdl.handle.net/20.500.12708/153453</div> </div>

http://hdl.handle.net/20.500.12708/153453

Harmonized surveillance of antimicrobial resistance (AMR) that also includes all potentially important environmental compartments is of major importance for tackling future challenges by supporting the reduction of AMR spreading over ecosystem boundaries. Even though numerous monitoring campaigns have investigated antibiotic resistant bacteria (ARBs) and antibiotic resistant genes (ARGs) in different natural and engineered environments, systematically collected data on their background concentrations in the environment are scarce. The aim of the presented study was to systematically determine the background contamination of soil, wastewater and surface waters with ARGs in Austria. Preliminary results of our comprehensive spatial & temporal monitoring already showed distinct patterns of ARGs and antibiotic concentrations in the different sample types investigated and could successfully profile their resistome. ARG profiling by high-throughput analysis (Resistomap) showed presence of 66/71 investigated genes in the first set of selected water samples. Investigated genes detected most frequently were integrons, followed by aminoglycosides and mobile genetic elements (MGE); detected least frequently were the taxonomic gene for enterococci and genes encoding vancomycin resistance. Quantification of seven ARGs (sul1, ermB, vanA, aph(3’)-IIa, aph(3’)-IIIa, tet(W), blaTEM-1) in different wastewater treatment plants (WWTPs) by qPCR showed very similar concentrations for influent samples in the same order of magnitude while they displayed somewhat higher variations for most ARGs in the effluents. Removal rates for most ARGs were in the range of 2 – 3 log steps, for ermB: 3 – 4 log steps. The results of the surface water samples were distributed over a wide range (partially up 5 log steps) due to the selection of the sites along a load gradient. In surface waters, detection rates and concentrations were mostly much lower than in WWTPs with sul1 and ermB detected most frequently and with the highest average concentrations (< 10^3 copies/ml). Temporal monitoring of wastewater influents and effluents generally showed only minor variations (within 1 log) over the course of one year, with some exceptions (for WWTP6: blaTEM-1 in influents and ermB in effluents). Interestingly, in contrast to effluent and environmental samples, the concentrations of ermB in WWTP influent samples were higher than those of sul1. Preliminary results from the investigations of ARGs in soil (by Resistomap & qPCR) revealed specific patterns according to soil type (agricultural, pristine, urban) and also impressively showed influence of fertilization on ARG soil profiles over time. ARG analysis is currently ongoing and results of the final sample set as well as of further ARGs will be presented at the conference. The final results of the presented project will help to identify environmental hot spots of AMR and to reduce the risk of transferring AMR from the environment to human and animal pathogens in line with the One Health approach. Furthermore, the findings will provide the basis for evidence-based advice to risk managers and possible routine ARG monitoring of the ecosystems studied in the future.

en

Antimicrobial resistance

Wastewater

Systematic Determination Of Antibiotic Resistance Genes In Soil, Wastewater And Surface Water In Austria

Presentation

Vortrag

Austrian Agency for Health and Food Safety, Austria

Poster Presentation

E4

C6

Environmental Monitoring and Climate Adaptation

Modeling and Simulation

50

50

E226-01 - Forschungsbereich Wassergütewirtschaft

E222-02 - Forschungsbereich Ingenieurhydrologie

E166-05-3 - Forschungsgruppe Mikrobiologie und Molekulare Diagnostik

0000-0002-9764-7534

0000-0001-5990-8329

0000-0002-9931-088X

0000-0002-6277-7048

0000-0002-7757-4163

0000-0002-6400-8218

6th International Symposium on the environmental dimension of antibiotic resistance

22-09-2022

27-09-2022

Hybrid

Event for scientific audience

Gothenburg

SE

Vierheilig, Julia

Online

Chemie

Umwelttechnik

Hydrologie

1040

2071

1053

50

30

20

en

conference poster not in proceedings

none

no Fulltext

Publications

http://purl.org/coar/resource_type/c_18co

E226-01 - Forschungsbereich Wassergütewirtschaft

E226-01 - Forschungsbereich Wassergütewirtschaft

E226-01 - Forschungsbereich Wassergütewirtschaft

E226-01 - Forschungsbereich Wassergütewirtschaft

Austrian Agency for Health and Food Safety, Austria

E226-01 - Forschungsbereich Wassergütewirtschaft

E222-02 - Forschungsbereich Ingenieurhydrologie

E166-05-3 - Forschungsgruppe Umweltmikrobiologie and Molekulare Diagnostik

E226-01 - Forschungsbereich Wassergütewirtschaft

E226-01 - Forschungsbereich Wassergütewirtschaft

0000-0002-9764-7534

0009-0004-6962-8370

0000-0001-5990-8329

0000-0002-9931-088X

0000-0002-6277-7048

0000-0002-7757-4163

0000-0002-6400-8218

E226 - Institut für Wassergüte und Ressourcenmanagement

E226 - Institut für Wassergüte und Ressourcenmanagement

E226 - Institut für Wassergüte und Ressourcenmanagement

E226 - Institut für Wassergüte und Ressourcenmanagement

E226 - Institut für Wassergüte und Ressourcenmanagement

E222 - Institut für Wasserbau und Ingenieurhydrologie

E166-05 - Forschungsbereich Biochemische Technologie

E226 - Institut für Wassergüte und Ressourcenmanagement

E226 - Institut für Wassergüte und Ressourcenmanagement