<div class="csl-bib-body">
<div class="csl-entry">Ellena, V., & Steiger, M. (2022, March). <i>The fungal sexual revolution continues: indications of sexuality in the citric acid producing fungus Aspergillus niger</i> [Poster Presentation]. 31st Fungal Genetics Conference, United States of America (the). http://hdl.handle.net/20.500.12708/153464</div>
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/153464
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dc.description.abstract
Sexual reproduction is an important proliferation mechanism which provides advantages to a species by promoting genetic variation. Interestingly, for a large group of fungal species, including the industrially relevant Aspergillus niger, this mode of reproduction has not been described yet. In recent years, strong indications of the sexual potential of A. niger have accumulated 1. These include the ability of certain strains to produce pre-mature sexual structures (sclerotia) and the presence of a mating type MAT1-1 locus in the commonly used A. niger strains, such as ATCC 1015 and CBS 513.88.
Here we show additional evidence of the sexual potential of A. niger. Formation of sclerotia could be induced in the progenitor of the industrial citric acid producing strains of A. niger, ATCC 1015, and in pyrG mutants derived from it 2. The capability of ATCC 1015 to form sclerotia, known to act as sexual structures in related organisms, represents a step forward towards the discovery of a sexual cycle in this important industrial species. To find the second mating type locus, we performed genome sequencing of the A. niger neotype strain CBS 554.65 and identified the missing MAT1-2 locus 3. A nucleotide alignment showed a different orientation of the MAT1-1 locus of ATCC 1015 compared to the MAT1-2 locus of CBS 554.65. While the genomic context of the MAT1-2 locus in CBS 554.65 is similar to the one of other MAT1-2 A. niger strains and other Aspergillus species, the region comprising the MAT1-1 locus is inverted in all sequenced strains of A. niger. These observations suggest the occurrence of genetic flipping or switching events at the MAT1-1 locus of A. niger, which might have a direct impact on its sexuality. These results provide new insights in the mating system of A. niger and pave the way for the discovery of a sexual cycle in a species long thought to be asexual.
1. Ellena, V., Sauer, M. & Steiger, M. G. Fungal Biol. Biotechnol. 7, 17 (2020).
2. Ellena, V., Bucchieri, D., Arcalis, E., Sauer, M. & Steiger, M. G. Fungal Biol. 125, 485–494 (2021).
3. Ellena, V., Seekles, S. J., Ram, A. F. J. & Steiger, M. G. BMC Genomics 1–17 (2021).
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dc.language.iso
en
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dc.subject
sexual cycle
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dc.subject
MAT genes
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dc.subject
sclerotia
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dc.title
The fungal sexual revolution continues: indications of sexuality in the citric acid producing fungus Aspergillus niger
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dc.type
Presentation
en
dc.type
Vortrag
de
dc.type.category
Poster Presentation
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tuw.researchTopic.id
M6
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tuw.researchTopic.id
E5
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tuw.researchTopic.id
E3
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tuw.researchTopic.name
Biological and Bioactive Materials
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tuw.researchTopic.name
Efficient Utilisation of Material Resources
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tuw.researchTopic.name
Climate Neutral, Renewable and Conventional Energy Supply Systems
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tuw.researchTopic.value
50
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tuw.researchTopic.value
25
-
tuw.researchTopic.value
25
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tuw.publication.orgunit
E166-05-2 - Forschungsgruppe Biochemie
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tuw.publication.orgunit
E166 - Institut für Verfahrenstechnik, Umwelttechnik und technische Biowissenschaften
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tuw.author.orcid
0000-0001-9781-434X
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tuw.author.orcid
0000-0002-8985-2343
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tuw.event.name
31st Fungal Genetics Conference
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tuw.event.startdate
15-03-2022
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tuw.event.enddate
20-03-2022
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tuw.event.online
Hybrid
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tuw.event.type
Event for scientific audience
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tuw.event.country
US
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tuw.event.institution
Genetics Society of America
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tuw.event.presenter
Ellena, Valeria
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tuw.event.track
Multi Track
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wb.sciencebranch
Chemische Verfahrenstechnik
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wb.sciencebranch
Biologie
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wb.sciencebranch
Industrielle Biotechnologie
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wb.sciencebranch.oefos
2040
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wb.sciencebranch.oefos
1060
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wb.sciencebranch.oefos
2090
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wb.sciencebranch.value
10
-
wb.sciencebranch.value
75
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wb.sciencebranch.value
15
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item.fulltext
no Fulltext
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item.cerifentitytype
Publications
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item.openairecristype
http://purl.org/coar/resource_type/c_18co
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item.languageiso639-1
en
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item.openairetype
conference poster not in proceedings
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item.grantfulltext
restricted
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crisitem.author.dept
E166-05-2 - Forschungsgruppe Mikrobiologie und Angewandte Genomik
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crisitem.author.orcid
0000-0002-8985-2343
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crisitem.author.parentorg
E166-05 - Forschungsbereich Biochemische Technologie