<div class="csl-bib-body">
<div class="csl-entry">Fritsche, S., & Steiger, M. (2022, March 15). <i>Aspergillus niger conidial germination: 3D live cell exploration</i> [Conference Presentation]. 31st Fungal Genetics Conference, Asilomar Conference Grounds, Pacific Grove, CA, United States of America (the). http://hdl.handle.net/20.500.12708/153808</div>
</div>
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/153808
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dc.description.abstract
Conidial germination describes the transition of dormant spores to hyphal structures. Breaking dormancy is followed by isotropic and polarized growth with a cell wall constantly being remodeled.
To explore and understand the rearrangement and timing of key events in A. niger, we use the Nanolive stain-free live cell imaging system - a combination of holography and tomography1. A temperature-controlled growth chamber is used for incubating the fungus on-stage of the microscope. With the 3D Nanoscopy technique, videos of the germination process can be recorded and morphological structures distinguished based on different refractive indices (RI). These structures provide a RI map of cellular details and 3D digital cell reconstruction revealed a finely resolved filamentous network. Overlay of fluorescent signals with the respective RI signal enables further identification of organelles for live, label- free observation in three dimensions.
en
dc.description.sponsorship
FFG - Österr. Forschungsförderungs- gesellschaft mbH
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dc.language.iso
en
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dc.subject
Microscopy
en
dc.subject
morphogenesis
en
dc.subject
live cell imaging
en
dc.title
Aspergillus niger conidial germination: 3D live cell exploration
en
dc.type
Presentation
en
dc.type
Vortrag
de
dc.relation.grantno
91110
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dc.type.category
Conference Presentation
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tuw.publication.invited
invited
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tuw.project.title
Erhöhung der zellulären Leistungsfähigkeit durch Reduktion von CO2 Emissionen
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tuw.researchTopic.id
M6
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tuw.researchTopic.id
E5
-
tuw.researchTopic.id
E3
-
tuw.researchTopic.name
Biological and Bioactive Materials
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tuw.researchTopic.name
Efficient Utilisation of Material Resources
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tuw.researchTopic.name
Climate Neutral, Renewable and Conventional Energy Supply Systems
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tuw.researchTopic.value
90
-
tuw.researchTopic.value
5
-
tuw.researchTopic.value
5
-
tuw.publication.orgunit
E166-05-2 - Forschungsgruppe Biochemie
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tuw.author.orcid
0000-0002-8202-6516
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tuw.author.orcid
0000-0002-8985-2343
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tuw.event.name
31st Fungal Genetics Conference
en
tuw.event.startdate
14-03-2022
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tuw.event.enddate
20-03-2022
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tuw.event.online
On Site
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tuw.event.type
Event for scientific audience
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tuw.event.place
Asilomar Conference Grounds, Pacific Grove, CA
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tuw.event.country
US
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tuw.event.presenter
Fritsche, Susanne
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tuw.event.track
Multi Track
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wb.sciencebranch
Chemische Verfahrenstechnik
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wb.sciencebranch
Biologie
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wb.sciencebranch
Industrielle Biotechnologie
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wb.sciencebranch.oefos
2040
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wb.sciencebranch.oefos
1060
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wb.sciencebranch.oefos
2090
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wb.sciencebranch.value
50
-
wb.sciencebranch.value
35
-
wb.sciencebranch.value
15
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item.languageiso639-1
en
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item.grantfulltext
restricted
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item.openairecristype
http://purl.org/coar/resource_type/c_18cp
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item.fulltext
no Fulltext
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item.openairetype
conference paper not in proceedings
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item.cerifentitytype
Publications
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crisitem.project.funder
FFG - Österr. Forschungsförderungs- gesellschaft mbH
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crisitem.project.grantno
91110
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crisitem.author.dept
E166-05-2 - Forschungsgruppe Mikrobiologie und Angewandte Genomik
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crisitem.author.orcid
0000-0002-8985-2343
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crisitem.author.parentorg
E166-05 - Forschungsbereich Biochemische Technologie
