<div class="csl-bib-body">
<div class="csl-entry">Akhgar, C. K., Ramer, G., Żbik, M., Trajnerowicz, A., Pawluczyk, J., Schwaighofer, A., & Lendl, B. (2020). The Next Generation of IR Spectroscopy : EC-QCL-Based Mid-IR Transmission Spectroscopy of Proteins with Balanced Detection. <i>Analytical Chemistry</i>, <i>92</i>(14), 9901–9907. https://doi.org/10.1021/acs.analchem.0c01406</div>
</div>
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dc.identifier.issn
0003-2700
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/15640
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dc.description.abstract
We report a mid-IR transmission setup for the analysis of the protein amide I and amide II band in aqueous solutions that achieves a limit of detection as low as 0.0025 mg mL–1 (outperforming our previous results and other state-of-the-art mid-IR-based techniques by almost an order of magnitude). This large improvement is made possible by combining the latest-generation external cavity-quantum cascade laser (EC-QCL) operated at room temperature with an optimized double-beam optical setup that adjusts the path length (26 μm) to ensure robust sample handling. For minimizing the noise introduced by the high-intensity laser light source, a thermoelectrically cooled mercury cadmium telluride balanced detection module was employed. In this way, noise levels better by a factor of up to 20 were achieved compared with single-channel measurements. Characteristic spectral features of proteins with different secondary structures were successfully identified at concentrations as low as 0.1 mg mL–1. Furthermore, a highly linear response was demonstrated for concentrations between 0.05 and 10 mg mL–1. The total acquisition time of the setup can be adapted to fulfill the required sensitivity of the protein measurements and to ensure maximum flexibility for future applications. The presented setup combines high sensitivity, large optical path lengths, and short measurement times and thus outperforms previous research type EC-QCL setups as well as commercially available instruments. This opens a wide range of future applications including protein–ligand interaction studies as well as qualitative and quantitative analyses of proteins in complex matrices such as those found in up- and downstream bioprocess monitoring and similar challenging applications which can not be readily met by conventional FT-IR spectroscopy.
en
dc.description.sponsorship
European Union’s Horizon 2020 ; Austrian Science Fund (FWF)
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dc.language.iso
en
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dc.publisher
AMER CHEMICAL SOC
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dc.relation.ispartof
Analytical Chemistry
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dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
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dc.subject
Amides
en
dc.subject
Absorption
en
dc.subject
Power
en
dc.subject
Spectroscopy
en
dc.subject
Lasers
en
dc.title
The Next Generation of IR Spectroscopy : EC-QCL-Based Mid-IR Transmission Spectroscopy of Proteins with Balanced Detection
en
dc.type
Article
en
dc.type
Artikel
de
dc.rights.license
Creative Commons Namensnennung 4.0 International
de
dc.rights.license
Creative Commons Attribution 4.0 International
en
dc.contributor.affiliation
Vigo System S.A., Polen
-
dc.contributor.affiliation
Vigo System S.A., Polen
-
dc.contributor.affiliation
Vigo System S.A., Polen
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dc.description.startpage
9901
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dc.description.endpage
9907
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dc.relation.grantno
818110
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dcterms.dateSubmitted
2020-04-01
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dc.type.category
Original Research Article
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tuw.container.volume
92
-
tuw.container.issue
14
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tuw.journal.peerreviewed
true
-
tuw.peerreviewed
true
-
tuw.version
vor
-
tuw.project.title
Fact-based personalised nutrition for the young
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dcterms.isPartOf.title
Analytical Chemistry
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tuw.publication.orgunit
E164 - Institut für Chemische Technologien und Analytik
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tuw.publisher.doi
10.1021/acs.analchem.0c01406
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dc.date.onlinefirst
2020-06-29
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dc.identifier.eissn
1520-6882
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dc.identifier.libraryid
AC17203691
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dc.description.numberOfPages
7
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tuw.author.orcid
0000-0001-8266-043X
-
tuw.author.orcid
0000-0001-8307-5435
-
tuw.author.orcid
0000-0003-2714-7056
-
tuw.author.orcid
0000-0003-3838-5842
-
dc.rights.identifier
CC BY 4.0
de
dc.rights.identifier
CC BY 4.0
en
item.openaccessfulltext
Open Access
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item.cerifentitytype
Publications
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item.languageiso639-1
en
-
item.openairecristype
http://purl.org/coar/resource_type/c_2df8fbb1
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item.grantfulltext
open
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item.fulltext
with Fulltext
-
item.openairetype
research article
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crisitem.author.dept
E164-02-1 - Forschungsgruppe Prozessanalytik
-
crisitem.author.dept
E164-02-1 - Forschungsgruppe Prozessanalytik
-
crisitem.author.dept
Vigo System S.A., Polen
-
crisitem.author.dept
Vigo System S.A., Polen
-
crisitem.author.dept
Vigo System S.A., Polen
-
crisitem.author.dept
E164-02-1 - Forschungsgruppe Prozessanalytik
-
crisitem.author.dept
E164-02 - Forschungsbereich Umwelt-, Prozessanalytik und Sensoren
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crisitem.author.orcid
0000-0001-8307-5435
-
crisitem.author.orcid
0000-0003-2714-7056
-
crisitem.author.orcid
0000-0003-3838-5842
-
crisitem.author.parentorg
E164-02 - Forschungsbereich Umwelt-, Prozessanalytik und Sensoren
-
crisitem.author.parentorg
E164-02 - Forschungsbereich Umwelt-, Prozessanalytik und Sensoren
-
crisitem.author.parentorg
E164-02 - Forschungsbereich Umwelt-, Prozessanalytik und Sensoren
-
crisitem.author.parentorg
E164 - Institut für Chemische Technologien und Analytik