<div class="csl-bib-body">
<div class="csl-entry">Schwaighofer, A., Alcaraz, M. R., Araman, C., Goicoechea, H., & Lendl, B. (2016). External cavity-quantum cascade laser infrared spectroscopy for secondary structure analysis of proteins at low concentrations. <i>Scientific Reports</i>. https://doi.org/10.1038/srep33556</div>
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Fourier transform infrared (FTIR) and circular dichroism (CD) spectroscopy are analytical techniques employed for the analysis of protein secondary structure. The use of CD spectroscopy is limited to low protein concentrations (<2 mg ml−1), while FTIR spectroscopy is commonly used in a higher concentration range (>5 mg ml−1). Here we introduce a quantum cascade laser (QCL)-based IR transmission setup for analysis of protein and polypeptide secondary structure at concentrations as low as 0.25 mg ml−1 in deuterated buffer solution. We present dynamic QCL-IR spectra of the temperature-induced α-helix to β-sheet transition of poly-L-lysine. The concentration dependence of the α-β transition temperature between 0.25 and 10 mg ml−1 was investigated by QCL-IR, FTIR and CD spectroscopy. By using QCL-IR spectroscopy it is possible to perform IR spectroscopic analysis in the same concentration range as CD spectroscopy, thus enabling a combined analysis of biomolecules secondary structure by CD and IR spectroscopy.
en
dc.description.sponsorship
Austrian research funding association (FFG)
-
dc.language
English
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dc.language.iso
en
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dc.publisher
Springer Nature
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dc.relation.ispartof
Scientific Reports
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dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
-
dc.subject
Bioanalytical chemistry
en
dc.subject
Infrared spectroscopy
en
dc.subject
Protein folding
en
dc.title
External cavity-quantum cascade laser infrared spectroscopy for secondary structure analysis of proteins at low concentrations
en
dc.type
Article
en
dc.type
Artikel
de
dc.rights.license
Creative Commons Namensnennung 4.0 International
de
dc.rights.license
Creative Commons Attribution 4.0 International
en
dc.contributor.affiliation
University of Vienna
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dc.contributor.affiliation
Ciudad Universitaria, Santa Fe, Argentina
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dc.relation.grantno
COMET 843546
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dc.rights.holder
The Author(s) 2016
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dc.type.category
Original Research Article
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tuw.journal.peerreviewed
true
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tuw.peerreviewed
true
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tuw.version
vor
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International Co-publication
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dcterms.isPartOf.title
Scientific Reports
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tuw.publication.orgunit
E164 - Institut für Chemische Technologien und Analytik
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tuw.publisher.doi
10.1038/srep33556
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dc.identifier.eissn
2045-2322
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dc.identifier.libraryid
AC11360473
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dc.description.numberOfPages
10
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dc.identifier.urn
urn:nbn:at:at-ubtuw:3-2117
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tuw.author.orcid
0000-0003-2714-7056
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tuw.author.orcid
0000-0003-3838-5842
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dc.rights.identifier
CC BY 4.0
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dc.rights.identifier
CC BY 4.0
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true
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en
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http://purl.org/coar/resource_type/c_2df8fbb1
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with Fulltext
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research article
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open
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Open Access
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Publications
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crisitem.author.dept
E164-02-1 - Forschungsgruppe Prozessanalytik
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crisitem.author.dept
E164 - Institut für Chemische Technologien und Analytik
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crisitem.author.dept
University of Vienna
-
crisitem.author.dept
Ciudad Universitaria, Santa Fe, Argentina
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crisitem.author.dept
E164-02 - Forschungsbereich Umwelt-, Prozessanalytik und Sensoren
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0000-0003-2714-7056
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0000-0003-3838-5842
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E164-02 - Forschungsbereich Umwelt-, Prozessanalytik und Sensoren
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E150 - Fakultät für Technische Chemie
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E164 - Institut für Chemische Technologien und Analytik