<div class="csl-bib-body">
<div class="csl-entry">Weiss, V., Subirats, X., Pickl-Herk, A., Bilek, G., Winkler, W., Kumar, M., Allmaier, G., Blaas, D., & Kenndler, E. (2012). Characterization of rhinovirus subviral A particles via capillary electrophoresis, electron microscopy and gas-phase electrophoretic mobility molecular analysis: Part I. <i>ELECTROPHORESIS</i>, <i>33</i>(12), 1833–1841. https://doi.org/10.1002/elps.201100647</div>
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dc.identifier.issn
0173-0835
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/164911
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dc.description.abstract
During infection, enteroviruses, such as human rhinoviruses (HRVs), convert from the native, infective form with a sedimentation coefficient of 150S to empty subviral particles sedimenting at 80S (B particles). B particles lack viral capsid protein 4 (VP4) and the single-stranded RNA genome. On the way to this end stage, a metastable intermediate particle is observed in the cell early after infection. This subviral A particle still contains the RNA but lacks VP4 and sediments at 135S. Native (150S) HRV serotype 2 (HRV2) as well as its empty (80S) capsid have been well characterized by capillary electrophoresis. In the present paper, we demonstrate separation of at least two forms of subviral A particles on the midway between native virions and empty 80S capsids by CE. For one of these intermediates, we established a reproducible way for its preparation and characterized this particle in terms of its electrophoretic mobility and its appearance in transmission electron microscopy (TEM). Furthermore, the conversion of this intermediate to 80S particles was investigated. Gas-phase electrophoretic mobility molecular analysis (GEMMA) yielded additional insights into sample composition. More data on particle characterization including its protein composition and RNA content (for unambiguous identification of the detected intermediate as subviral A particle) will be presented in the second part of the publication.
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dc.language.iso
en
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dc.publisher
WILEY
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dc.relation.ispartof
ELECTROPHORESIS
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dc.subject
Biochemistry
en
dc.subject
Analytical Chemistry
en
dc.subject
Clinical Biochemistry
en
dc.title
Characterization of rhinovirus subviral A particles via capillary electrophoresis, electron microscopy and gas-phase electrophoretic mobility molecular analysis: Part I
en
dc.type
Artikel
de
dc.type
Article
en
dc.description.startpage
1833
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dc.description.endpage
1841
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dc.type.category
Original Research Article
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tuw.container.volume
33
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tuw.container.issue
12
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tuw.journal.peerreviewed
true
-
tuw.peerreviewed
true
-
tuw.researchTopic.id
M6
-
tuw.researchTopic.name
Biological and Bioactive Materials
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tuw.researchTopic.value
100
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dcterms.isPartOf.title
ELECTROPHORESIS
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tuw.publication.orgunit
E164-01-1 - Forschungsgruppe Massenspektrometrische Bio- und Polymeranalytik
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tuw.publisher.doi
10.1002/elps.201100647
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dc.date.onlinefirst
2012-06-28
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dc.identifier.eissn
1522-2683
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dc.description.numberOfPages
9
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tuw.author.orcid
0000-0002-0056-6819
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wb.sci
true
-
wb.sciencebranch
Chemie
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wb.sciencebranch
Hygiene, medizinische Mikrobiologie
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wb.sciencebranch.oefos
13
-
wb.sciencebranch.oefos
34
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wb.facultyfocus
Bioscience Technology
de
wb.facultyfocus
Bioscience Technology
en
wb.facultyfocus.faculty
E150
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item.fulltext
no Fulltext
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item.languageiso639-1
en
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item.openairecristype
http://purl.org/coar/resource_type/c_2df8fbb1
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item.openairetype
research article
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item.grantfulltext
none
-
item.cerifentitytype
Publications
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crisitem.author.dept
E164-01-1 - Forschungsgruppe Massenspektrometrische Bio- und Polymeranalytik
-
crisitem.author.dept
E164 - Institut für Chemische Technologien und Analytik
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crisitem.author.dept
Universidad Politécnica de Madrid, Spain
-
crisitem.author.dept
E164-01-1 - Forschungsgruppe Massenspektrometrische Bio- und Polymeranalytik
-
crisitem.author.orcid
0000-0002-0056-6819
-
crisitem.author.orcid
0000-0002-1438-9462
-
crisitem.author.parentorg
E164-01 - Forschungsbereich Imaging und Instrumentelle Analytische Chemie
-
crisitem.author.parentorg
E150 - Fakultät für Technische Chemie
-
crisitem.author.parentorg
E164-01 - Forschungsbereich Imaging und Instrumentelle Analytische Chemie