DC FieldValueLanguage
dc.contributor.authorArslan, Aysu-
dc.contributor.authorSteiger, Wolfgang-
dc.contributor.authorRoose, Patrice-
dc.contributor.authorVan den Bergen, Hugues-
dc.contributor.authorGruber, Peter-
dc.contributor.authorZerobin, Elise-
dc.contributor.authorGantner, Franziska-
dc.contributor.authorGuillaume, Olivier-
dc.contributor.authorOvsianikov, Aleksandr-
dc.contributor.authorVan Vlierberghe, Sandra-
dc.contributor.authorDubruel, Peter-
dc.date.accessioned2021-02-15T08:36:17Z-
dc.date.available2021-02-15T08:36:17Z-
dc.date.issued2020-
dc.identifier.issn1369-7021-
dc.identifier.urihttp://hdl.handle.net/20.500.12708/16841-
dc.description.abstractTwo-photon polymerization (2PP) is a high-resolution 3D-printing technology with a very rapidly expanding field of applications, including tissue engineering (TE). In this field, 2PP offers unprecedented possibilities for systematic studies of both cell–cell and cell–material interactions in 3D. For TE applications, the reliable production of biodegradable micro-scaffolds in porous, complex architectures is essential. However, the number of biodegradable materials that support the required level of spatial resolution is very limited, being a major bottleneck for the use of 2PP in the TE field. Herein, we introduce a hexa-functional urethane-based biodegradable precursor that overcomes the limitations associated with the high-resolution printing of current biodegradable precursors. The precursor is a telechelic urethane-based poly-ε-caprolactone (PCL) possessing three acrylate functionalities at each polymer end group which enables the reliable production of complex architectures owing to its superior physical properties as compared to the traditional di-acrylate terminated analogs. The newly developed hexa-functional telechelic urethane-based PCL reveals enhanced crosslinking kinetics and one order of magnitude higher Young's modulus compared to the di-functional precursor (57.8 versus 6.3 MPa), providing an efficient and solvent-free 2PP processing at fast scanning speeds of up to 100 mm s−1 with unprecedented feature resolutions (143 ± 18 nm at 100 mm s−1 scanning speed). The crosslinked hexa-functional polymer combines strength and flexibility owing to the segregation between its hard polyacrylate and soft PCL segments, which makes it suitable for biological systems in contrast to the highly crosslinked and rigid structures typically manufactured by 2PP. Furthermore, it revealed lower degradation rate compared to its di-functional analog, which can be considered as an advantage in terms of biocompatibility due to the slower formation of acidic degradation products. Extracts of the developed polymers did not show a cytotoxic effect on the L929 fibroblasts as confirmed via ISO 10993-5 standard protocol. The presented precursor design constitutes a simple and effective approach that can be easily translated towards other biodegradable polymers for the manufacturing of biodegradable constructs with nano-scale precision, offering for the first time to use the true capabilities of 2PP for TE applications with the use of synthetic biodegradable polymers.en
dc.description.sponsorshipAustrian Science Fund (FWF)-
dc.description.sponsorshipEuropean Research Council-
dc.language.isoen-
dc.publisherELSEVIER SCI LTD-
dc.relation.ispartofMaterials Today-
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/-
dc.subjecttissue engineeringen
dc.subjecttwo-photon polymerization (2PP)en
dc.titlePolymer architecture as key to unprecedented high-resolution 3D-printing performance : The case of biodegradable hexa-functional telechelic urethane-based poly-ε-caprolactoneen
dc.typeArticleen
dc.typeArtikelde
dc.rights.licenseCreative Commons Namensnennung 4.0 Internationalde
dc.rights.licenseCreative Commons Attribution 4.0 Internationalen
dc.identifier.scopus2-s2.0-85097774335-
dc.identifier.urlhttps://api.elsevier.com/content/abstract/scopus_id/85097774335-
dc.contributor.affiliationGhent University, Belgium-
dc.contributor.affiliationAustrian Cluster for Tissue Regeneration, Austria-
dc.contributor.affiliationAllnex Belgium SA/NV, Belgium-
dc.contributor.affiliationAustrian Cluster for Tissue Regeneration, Austria-
dc.contributor.affiliationGhent University, Belgium-
dc.contributor.affiliationGhent University, Belgium-
dc.relation.grantnoI 2444-
dc.relation.grantno772464-
dc.type.categoryOriginal Research Article-
tuw.journal.peerreviewedtrue-
tuw.peerreviewedtrue-
tuw.versionvor-
tuw.project.titleNeuartige zell-instruktive Hydrogele für laser-basiertes hochauflösendes 3D Drucken-
tuw.project.titleThird Strategy in Tissue Engineering – Functional microfabricated multicellular spheroid carriers for tissue engineering and regeneration-
dcterms.isPartOf.titleMaterials Today-
tuw.publication.orgunitE308-02-3 - Forschungsgruppe 3D Printing and Biofabrication-
tuw.publisher.doi10.1016/j.mattod.2020.10.005-
dc.date.onlinefirst2020-12-13-
dc.identifier.eissn1873-4103-
dc.identifier.libraryidAC16151785-
dc.description.numberOfPages15-
tuw.author.orcid0000-0001-7161-4171-
tuw.author.orcid0000-0003-0735-113X-
tuw.author.orcid0000-0001-5846-0198-
dc.rights.identifierCC BY 4.0de
dc.rights.identifierCC BY 4.0en
item.openairetypeArticle-
item.openairetypeArtikel-
item.openaccessfulltextOpen Access-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextopen-
item.fulltextwith Fulltext-
item.cerifentitytypePublications-
item.cerifentitytypePublications-
crisitem.author.deptGhent University, Belgium-
crisitem.author.deptAustrian Cluster for Tissue Regeneration, Austria-
crisitem.author.deptAllnex Belgium SA/NV, Belgium-
crisitem.author.deptE308-02-3 - Forschungsgruppe 3D Printing and Biofabrication-
crisitem.author.orcid0000-0001-7161-4171-
crisitem.author.orcid0000-0003-0735-113X-
crisitem.author.orcid0000-0001-5846-0198-
crisitem.author.parentorgE308-02 - Forschungsbereich Polymer- und Verbundwerkstoffe-
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