<div class="csl-bib-body">
<div class="csl-entry">Romann, P., Kolar, J., Chappuis, L., Herwig, C., Villiger, T. K., & Bielser, J.-M. (2023). Maximizing yield of perfusion cell culture processes: Evaluation and scale-up of continuous bleed recycling. <i>Biochemical Engineering Journal</i>, <i>193</i>, Article 108873. https://doi.org/10.1016/j.bej.2023.108873</div>
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dc.identifier.issn
1369-703X
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/171452
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dc.description.abstract
Bleed recycling is a novel method to increase the yield of steady-state perfusion processes by concentrating process bleed to selectively remove biomass and recycle the liquid fraction. This results in significant product saving which otherwise would go to waste. As long as cells can be concentrated and separated, existing cell separation devices can be used for such an application. However, limited information comparing operation modes and efficiency for bleed recycling applications is available. For the first time, inclined gravity settling has been used as bleed recycling technology and was compared to acoustic separation. Except for lower debris removal, inclined gravity settling showed similar bleed recycling efficiency and no negative impact on cell viabilities, nutrient and metabolite levels and product quality. Additionally considering reduced system complexity and facilitated scale-up, inclined gravity settling was the preferred technology for further evaluation during a 42-day lab-scale perfusion process. Up to a 3.5-fold bleed reduction and an average harvest rate increase of 19% was achieved. Scalability was subsequently tested with a large-scale inclined gravity settler suitable for a 2000 L perfusion process confirming performance of lab-scale experiments. Bleed recycling characterization data from screening experiments combined with scalability demonstration facilitates decision making when considering bleed recycling for novel perfusion process settings to reduce perfusion waste, increase process sustainability and boost overall process yield.
en
dc.language.iso
en
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dc.publisher
Elsevier
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dc.relation.ispartof
Biochemical Engineering Journal
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dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
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dc.subject
Blled Recycling
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dc.subject
Perfusion
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dc.subject
Integrated Continuous Biomanufacturing
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dc.title
Maximizing yield of perfusion cell culture processes: Evaluation and scale-up of continuous bleed recycling
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dc.type
Article
en
dc.type
Artikel
de
dc.rights.license
Creative Commons Namensnennung 4.0 International
de
dc.rights.license
Creative Commons Attribution 4.0 International
en
dc.contributor.affiliation
University of Applied Sciences and Arts Northwestern Switzerland, Switzerland
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dc.contributor.affiliation
University of Applied Sciences and Arts Northwestern Switzerland, Switzerland
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dc.contributor.affiliation
Biotech Process Sciences, Switzerland
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dc.contributor.affiliation
University of Applied Sciences and Arts Northwestern Switzerland, Switzerland