<div class="csl-bib-body">
<div class="csl-entry">Koizar, D. (2021). <i>Characterization of the phenotypic diversity of Komagataella strains for biotechnological applications</i> [Diploma Thesis, Technische Universität Wien]. reposiTUm. https://doi.org/10.34726/hss.2021.55486</div>
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dc.identifier.uri
https://doi.org/10.34726/hss.2021.55486
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/17604
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dc.description
Abweichender Titel nach Übersetzung der Verfasserin/des Verfassers
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dc.description.abstract
The methylotrophic yeast Pichia pastoris is an important model organism for heterologous protein production. It belongs to the genus Komagataella, which is now divided into seven different Komagataella species. The main focus of this thesis was the characterization of growth and mating behavior of 25 different Komagataella strains. The growth characterization was realized with spotting assays using various carbon sources and stressors at two different temperatures, which gives a good selection of strains with useful properties for further analysis. Not only growth behavior but also mating between different strains and species is not well studied for Komagataella. For the mating experiments, strains were transformed with a vector containing antibiotic resistance and a reporter protein, either geneticin/eGFP or nourseothricin/mCherry. Characterization of mating and the ability to undergo meiosis is essential for strain characterization, especially in terms of species definition but also for biotechnological applications. Based on the obtained results, two strains with differences in xylose utilization were mated, and growth curves of the F1 generation were recorded. This small-scale experiment showed the possibility to create descendants harboring the benefits of both parents as well as the analysis of complex traits and their genetic background.
en
dc.language
English
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dc.language.iso
en
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dc.rights.uri
http://rightsstatements.org/vocab/InC/1.0/
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dc.subject
CRISPR-Cas9
en
dc.subject
fluorescence microscopy
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dc.subject
Phenotyping and genotyping sequence analysis
en
dc.title
Characterization of the phenotypic diversity of Komagataella strains for biotechnological applications
en
dc.title.alternative
Charakterisierung der phänotypischen Vielfalt von Komagataella-Stämmen für biotechnologische Anwendungen
de
dc.type
Thesis
en
dc.type
Hochschulschrift
de
dc.rights.license
In Copyright
en
dc.rights.license
Urheberrechtsschutz
de
dc.identifier.doi
10.34726/hss.2021.55486
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dc.contributor.affiliation
TU Wien, Österreich
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dc.rights.holder
Daniel Koizar
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dc.publisher.place
Wien
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tuw.version
vor
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tuw.thesisinformation
Technische Universität Wien
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tuw.publication.orgunit
E166 - Institut für Verfahrenstechnik, Umwelttechnik und technische Biowissenschaften