<div class="csl-bib-body">
<div class="csl-entry">Besleaga, M., Vignolle, G. A., Kopp, J., Spadiut, O., Mach, R. L., Mach-Aigner, A. R., & Zimmermann, C. (2023). Evaluation of reference genes for transcript analyses in Komagataella phaffii (Pichia pastoris). <i>Fungal Biology and Biotechnology</i>, <i>10</i>, Article 7. https://doi.org/10.1186/s40694-023-00154-1</div>
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/177383
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dc.description.abstract
The yeast Komagataella phaffii (Pichia pastoris) is routinely used for heterologous protein expression and is suggested as a model organism for yeast. Despite its importance and application potential, no reference gene for transcript analysis via RT-qPCR assays has been evaluated to date. In this study, we searched publicly available RNASeq data for stably expressed genes to find potential reference genes for relative transcript analysis by RT-qPCR in K. phaffii. To evaluate the applicability of these genes, we used a diverse set of samples from three different strains and a broad range of cultivation conditions. The transcript levels of 9 genes were measured and compared using commonly applied bioinformatic tools.
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dc.description.sponsorship
FWF Fonds zur Förderung der wissenschaftlichen Forschung (FWF)
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dc.description.sponsorship
FFG - Österr. Forschungsförderungs- gesellschaft mbH
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dc.language.iso
en
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dc.relation.ispartof
Fungal Biology and Biotechnology
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dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
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dc.subject
Housekeeping genes
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dc.subject
Komagataella phaffii
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dc.subject
Pichia pastoris
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dc.subject
RT-qPCR
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dc.subject
Relative transcript analysis
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dc.subject
Transcriptomics
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dc.title
Evaluation of reference genes for transcript analyses in Komagataella phaffii (Pichia pastoris)
