<div class="csl-bib-body">
<div class="csl-entry">Karimi Aghcheh, R. (2009). <i>LAE1 : a regulator of Trichoderma reesei secondary metabolism and sporulation</i> [Diploma Thesis, Technische Universität Wien]. reposiTUm. http://hdl.handle.net/20.500.12708/186629</div>
</div>
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/186629
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dc.description.abstract
The filamentous fungus Trichoderma reesei is well known for its production different types of peptaibols which are considered a unique class of antibiotics being rich in the non-proteinogenic alfa-aminoisobutyric acid (Aib) and having an N-acyl (usually acetyl) terminus, and a C-terminal amino alcohol, such as phenylalaninol or leucinol. Production of peptaibols is still hampered by the poor information about the regulation of their biosynthesis, however. In Aspergillus spp., the laeA (loss of aflR expression) gene encodes a protein-methyltransferase that acts as global regulator of secondary metabolism. In this study, the LaeA orthologue of T. reesei (Lae1) has been identified by a phylogenetic approach. To elucidate a possible regulation of T. reesei peptaibol production by LAE1, we deleted the lae1 gene. To this end, a knock-out vector containing 1kb upstream and 1kb downstream non-coding regions of lae1 interrupted by the pyr4 (encoding the orotidine 5'-monophosphate decarboxylase required for pyrimidine biosynthesis) selection marker was constructed.<br />Transformation of the delta-lae1pyr4 deletion cassette into the KU70 negative T. reesei strain resulted in a number of lae1 deleted strains which was confirmed by diagnostic PCR. Analysis of the phenotype of these strains showed that LAE1 has a positive regulatory role in pigment formation and sporulation. Intact-cell Matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis of strains deleted in lae1, showed an impairment of formation of the 20-residues long peptaibols, from the paracelsins type, and of the 11-residues peptaibols, from the trichovorin/trickorozin type in the delta lae1 strains. The expression level of tpc1 and tps1 genes encoding peptaibol synthase of the paracelsin type and of the trichovorin type respectively showed that the lae1 knock-out causes partial loss of tpc1 and tps1 transcription in comparison with the parent strain. The results suggest that the LAE1, could considered as a regulator of the expression of secondary metabolite genes including peptaibols and sporulation.
en
dc.language
English
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dc.language.iso
en
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dc.subject
Lae1
de
dc.subject
Trichoderma
de
dc.subject
reesei
de
dc.subject
sekundär
de
dc.subject
Metabolismus
de
dc.subject
Sporulation
de
dc.subject
Peptaibol
de
dc.subject
Lae1
en
dc.subject
Trichoderma
en
dc.subject
reesei
en
dc.subject
secondary
en
dc.subject
metabolism
en
dc.subject
sporulation
en
dc.subject
peptaibol
en
dc.title
LAE1 : a regulator of Trichoderma reesei secondary metabolism and sporulation
en
dc.type
Thesis
en
dc.type
Hochschulschrift
de
dc.contributor.affiliation
TU Wien, Österreich
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tuw.thesisinformation
Technische Universität Wien
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dc.contributor.assistant
Seiboth, Bernhard
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tuw.publication.orgunit
E166 - Institut für Verfahrenstechnik, Umwelttechnik und Technische Biowissenschaften
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dc.type.qualificationlevel
Diploma
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dc.identifier.libraryid
AC07806353
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dc.description.numberOfPages
60
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dc.thesistype
Diplomarbeit
de
dc.thesistype
Diploma Thesis
en
tuw.advisor.staffStatus
staff
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tuw.assistant.staffStatus
staff
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item.languageiso639-1
en
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item.cerifentitytype
Publications
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item.cerifentitytype
Publications
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item.openairecristype
http://purl.org/coar/resource_type/c_18cf
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item.openairecristype
http://purl.org/coar/resource_type/c_18cf
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item.fulltext
no Fulltext
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item.grantfulltext
none
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item.openairetype
Thesis
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item.openairetype
Hochschulschrift
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crisitem.author.dept
E166 - Institut für Verfahrenstechnik, Umwelttechnik und technische Biowissenschaften