<div class="csl-bib-body">
<div class="csl-entry">Honeder, S., Liesinger, L., Gindlhuber, J., Tomin, T., Schittmayer-Schantl, M., Brcic, L., Lindenmann, J., & Birner-Grünberger, R. (2023, July 30). <i>Activity-based proteomic profiling reveals reduction of lipid hydrolase activity levels in lung tumors</i> [Poster Presentation]. FEBS 2023 Advanced Course: 15th European Summer School Advanced Proteomics, Brixen, Italy. http://hdl.handle.net/20.500.12708/188135</div>
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/188135
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dc.description.abstract
Lipids are crucial for maintaining membrane homeostasis and many other functions of healthy cells. A sufficient supply of lipids is essential for rapidly proliferating cells. Cancer cells frequently show increased uptake of lipids, making lipid metabolism a promising therapeutic target in cancer. More recently, the accumulation of neutral lipids like triacylglycerol and cholesterol esters in lipid droplets has evolved as a hallmark of aggressive cancers. In addition to increased uptake and biosynthesis of lipids, a decrease in intracellular lipid hydrolysis contributes to lipid droplet accumulation. However, lipid hydrolases' role in the cancer context is less widely explored.
By employing state-of-the-art mass spectrometry techniques, we aimed to explore lipid hydrolysis in non-small cell lung cancer (NSCLC) on the proteome, lipidome, and serine hydrolase activity level. Tumors and normal tumor-adjacent (NTA) tissue of NSCLC patients were collected and subjected to in-depth proteomics, lipidomics, and activity-based proteomic profiling (ABPP). Tissues were collected immediately after tumor resection and flash-frozen. In the case of ABPP, tissues were immediately incubated with a small serine hydrolase-specific probe recognized by active serine hydrolases. Labeling active serine hydrolases with the probe allowed us to enrich active enzymes belonging to this class of enzymes (including lipases) and subsequently quantify the abundance of active enzymes. Through ABPP, we identified several serine hydrolases with higher activities (fold change > 1.5) in healthy tissue, including monoglyceride lipase (MGL), neuropathy target esterase (PNPLA6), epoxide hydrolase (EPHX1), neutral cholesterol ester hydrolase (NCEH1) and liver carboxylesterase 1 (CES1). Proteomics analysis revealed that several of those lipid hydrolases, as well as several fatty acid-binding proteins, are also higher in abundance in NTA tissue. Furthermore, lipidomics analysis on the same tissues exposed significant triglyceride accumulation and higher levels of ceramides and lysophosphatidylcholines in tumors.
Collectively, these data highlight the implication of lipid hydrolysis in NSCLC and suggest that lung cancer cells shut down lipid catabolism pathways contributing to the observed changes in the lipidome.
en
dc.description.sponsorship
FWF Fonds zur Förderung der wissenschaftlichen Forschung (FWF)
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dc.language.iso
en
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dc.subject
lung cancer
en
dc.subject
activity-based proteomics
en
dc.subject
lipidomics
en
dc.title
Activity-based proteomic profiling reveals reduction of lipid hydrolase activity levels in lung tumors
en
dc.type
Presentation
en
dc.type
Vortrag
de
dc.contributor.affiliation
Medical University of Graz, Austria
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dc.contributor.affiliation
Medical University of Graz, Austria
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dc.contributor.affiliation
Medical University of Graz, Austria
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dc.contributor.affiliation
Medical University of Graz, Austria
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dc.relation.grantno
F 7309-B21
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dc.type.category
Poster Presentation
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tuw.project.title
Lipidhydrolyse im Krebs und in Lipid-assoziierten Krankheiten
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tuw.researchTopic.id
M6
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tuw.researchTopic.name
Biological and Bioactive Materials
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tuw.researchTopic.value
100
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tuw.publication.orgunit
E164-01-3 - Forschungsgruppe Bioanalytik
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tuw.author.orcid
0000-0002-7201-6293
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tuw.author.orcid
0000-0002-6155-5208
-
tuw.author.orcid
0000-0002-7071-2316
-
tuw.author.orcid
0000-0002-9098-8416
-
tuw.author.orcid
0000-0003-1276-7666
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tuw.author.orcid
0000-0003-3950-0312
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tuw.event.name
FEBS 2023 Advanced Course: 15th European Summer School Advanced Proteomics
en
dc.description.sponsorshipexternal
FWF
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dc.relation.grantnoexternal
W1226
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tuw.event.startdate
30-07-2023
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tuw.event.enddate
05-08-2023
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tuw.event.online
On Site
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tuw.event.type
Event for scientific audience
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tuw.event.place
Brixen
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tuw.event.country
IT
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tuw.event.presenter
Honeder, Sophie
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tuw.event.track
Single Track
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wb.sciencebranch
Chemie
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wb.sciencebranch
Pharmazie, Pharmakologie, Toxikologie
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wb.sciencebranch
Anatomie, Pathologie, Physiologie
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wb.sciencebranch.oefos
1040
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wb.sciencebranch.oefos
3012
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wb.sciencebranch.oefos
3011
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wb.sciencebranch.value
50
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wb.sciencebranch.value
20
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wb.sciencebranch.value
30
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item.languageiso639-1
en
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item.openairecristype
http://purl.org/coar/resource_type/c_18cf
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item.openairecristype
http://purl.org/coar/resource_type/c_18cf
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item.fulltext
no Fulltext
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item.grantfulltext
restricted
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item.cerifentitytype
Publications
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item.cerifentitytype
Publications
-
item.openairetype
Presentation
-
item.openairetype
Vortrag
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crisitem.author.dept
Medical University of Graz, Austria
-
crisitem.author.dept
E164-01-3 - Forschungsgruppe Bioanalytik
-
crisitem.author.dept
Medical University of Graz, Austria
-
crisitem.author.dept
E164-01-3 - Forschungsgruppe Bioanalytik
-
crisitem.author.dept
E164-01-3 - Forschungsgruppe Bioanalytik
-
crisitem.author.dept
Medical University of Graz, Austria
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crisitem.author.dept
Medical University of Graz, Austria
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crisitem.author.dept
E164-01 - Forschungsbereich Imaging und Instrumentelle Analytische Chemie
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crisitem.author.orcid
0000-0002-7201-6293
-
crisitem.author.orcid
0000-0002-6155-5208
-
crisitem.author.orcid
0000-0002-7071-2316
-
crisitem.author.orcid
0000-0002-9098-8416
-
crisitem.author.orcid
0000-0003-1276-7666
-
crisitem.author.orcid
0000-0003-3950-0312
-
crisitem.author.parentorg
E164-01 - Forschungsbereich Imaging und Instrumentelle Analytische Chemie
-
crisitem.author.parentorg
E164-01 - Forschungsbereich Imaging und Instrumentelle Analytische Chemie
-
crisitem.author.parentorg
E164-01 - Forschungsbereich Imaging und Instrumentelle Analytische Chemie
-
crisitem.author.parentorg
E164 - Institut für Chemische Technologien und Analytik
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crisitem.project.funder
FWF Fonds zur Förderung der wissenschaftlichen Forschung (FWF)