reposiTUm: Multi-omics analysis reveals LCK as a key player of invasiveness and plasticity in human oral cancer

DC Field

Value

Language

dc.contributor.author

Pyko, Jonas

dc.contributor.author

Tomin, Tamara

dc.contributor.author

Müller, Lisa

dc.contributor.author

Kappler, Matthias

dc.contributor.author

Eckert, Alexander W.

dc.contributor.author

Glaß, Markus

dc.contributor.author

Misiak, Danny

dc.contributor.author

Schinagl, Maximilian

dc.contributor.author

Schittmayer, Matthias

dc.contributor.author

Hüttelmaier, Stefan

dc.contributor.author

Hatzfeld, Mechthild

dc.contributor.author

Haemmerle, Monika

dc.contributor.author

Birner-Grünberger, Ruth

dc.contributor.author

Gutschner, Tony

dc.date.accessioned

2023-10-18T11:45:58Z

dc.date.available

2023-10-18T11:45:58Z

dc.date.issued

2023-10-03

dc.identifier.citation

<div class="csl-bib-body"> <div class="csl-entry">Pyko, J., Tomin, T., Müller, L., Kappler, M., Eckert, A. W., Glaß, M., Misiak, D., Schinagl, M., Schittmayer, M., Hüttelmaier, S., Hatzfeld, M., Haemmerle, M., Birner-Grünberger, R., & Gutschner, T. (2023, October 3). <i>Multi-omics analysis reveals LCK as a key player of invasiveness and plasticity in human oral cancer</i> [Poster Presentation]. Tumor Heterogeneity, Plasticity and Therapy (2nd edition), Leuven, Belgium. http://hdl.handle.net/20.500.12708/189072</div> </div>

dc.identifier.uri

http://hdl.handle.net/20.500.12708/189072

dc.description.abstract

Patients diagnosed with advanced metastatic oral cancer have a 5-year survival rate of 30%. Although metastases are the main cause of cancer lethality, efficient treatments are still lacking (Ganesh & Massagué, 2021). Importantly, phenotypic plasticity markedly influences the metastatic progression, as it enables tumour cells to tolerate and adapt to several different stress factors and changing environments. Moreover, clonal heterogeneity accelerates metastasis dissemination by increasing the probability for invasive subclones. By exploiting the intratumoural heterogeneity of oral cancer cells (SAS), we recently identified the lymphocyte cell-specific protein-tyrosine kinase (LCK) as a driver of invasion and migration (Weiße et al., 2021). Importantly, inhibition of LCK induced cell differentiation and reduced the motility of high invasive SAS subclones. In order to further characterize the mechanisms of invasion and to identify the molecular targets of LCK, we analyzed the proteome, lipidome and metabolome of high and low invasive SAS clones. Intriguingly, key enzymes of the cholesterol synthesis pathway are downregulated in highly invasive clones. Additionally, we observed a switch from free fatty acids to carnitines and phospholipids and a loss of lipid storage capacity. Interestingly, PPARG, a key regulator of fatty acid metabolism, is strongly co-regulated with LCK. Inhibition of PPARG increases the lipid storage and reduces the motility of SAS cells, thereby presenting a potential link between LCK and the observed effects. Our work emphasizes fatty acid and membrane lipid reorganization as an important molecular mechanism involved in oral cancer cell plasticity and invasion into the surrounding tissue.

dc.description.sponsorship

FWF Fonds zur Förderung der wissenschaftlichen Forschung (FWF)

dc.language.iso

dc.subject

cancer

dc.subject

multi-omics

dc.subject

Lck

dc.title

Multi-omics analysis reveals LCK as a key player of invasiveness and plasticity in human oral cancer

dc.type

Presentation

dc.type

Vortrag

dc.contributor.affiliation

Martin Luther University Halle-Wittenberg, Germany

dc.contributor.affiliation

Martin Luther University Halle-Wittenberg, Germany

dc.contributor.affiliation

Martin Luther University Halle-Wittenberg, Germany

dc.contributor.affiliation

Paracelsus Medical University, Austria

dc.contributor.affiliation

Martin Luther University Halle-Wittenberg, Germany

dc.contributor.affiliation

Martin Luther University Halle-Wittenberg, Germany

dc.contributor.affiliation

TU Wien, Austria

dc.contributor.affiliation

Martin Luther University Halle-Wittenberg, Germany

dc.contributor.affiliation

Martin Luther University Halle-Wittenberg, Germany

dc.contributor.affiliation

University Hospital in Halle, Germany

dc.relation.grantno

F 7309-B21

dc.type.category

Poster Presentation

tuw.project.title

Lipidhydrolyse im Krebs und in Lipid-assoziierten Krankheiten

tuw.researchTopic.id

tuw.researchTopic.name

Biological and Bioactive Materials

tuw.researchTopic.value

100

tuw.linking

https://www.vibconferences.be/events/tumor-heterogeneity-plasticity-and-therapy-2nd-edition

tuw.publication.orgunit

E164-01-3 - Forschungsgruppe Bioanalytik

tuw.author.orcid

0009-0003-0589-5157

tuw.author.orcid

0000-0002-7071-2316

tuw.author.orcid

0000-0001-5931-8425

tuw.author.orcid

0000-0003-1009-7208

tuw.author.orcid

0000-0003-3249-655X

tuw.author.orcid

0000-0001-9335-4227

tuw.author.orcid

0000-0001-9968-7073

tuw.author.orcid

0000-0003-3145-0541

tuw.author.orcid

0000-0003-3950-0312

tuw.author.orcid

0000-0002-1722-3134

tuw.event.name

Tumor Heterogeneity, Plasticity and Therapy (2nd edition)

tuw.event.startdate

03-10-2023

tuw.event.enddate

04-10-2023

tuw.event.online

On Site

tuw.event.type

Event for scientific audience

tuw.event.place

Leuven

tuw.event.country

tuw.event.presenter

Pyko, Jonas

tuw.event.track

Single Track

wb.sciencebranch

Chemie

wb.sciencebranch

Biologie

wb.sciencebranch

Anatomie, Pathologie, Physiologie

wb.sciencebranch.oefos

1040

wb.sciencebranch.oefos

1060

wb.sciencebranch.oefos

3011

wb.sciencebranch.value

item.cerifentitytype

Publications

item.languageiso639-1

item.fulltext

no Fulltext

item.openairetype

conference poster not in proceedings

item.openairecristype

http://purl.org/coar/resource_type/c_18co

item.grantfulltext

none

crisitem.project.funder

FWF - Österr. Wissenschaftsfonds

crisitem.project.grantno

F 7309-B21

crisitem.author.dept