Schandl, Stephan

Osondu-Chuka, Goodness

Reimhult, Erik

Guillaume, Olivier

2024-01-03T15:14:54Z

2024-01-03T15:14:54Z

2023-01-16

<div class="csl-bib-body"> <div class="csl-entry">Schandl, S., Osondu-Chuka, G., Reimhult, E., &#38; Guillaume, O. (2023, January 16). <i>The Influence of ACetylation on the Interachtion between Alginate and Tobramycin</i> [Poster Presentation]. Break Biofilms Workshop 2023, Wien, Austria. http://hdl.handle.net/20.500.12708/191021</div> </div>

http://hdl.handle.net/20.500.12708/191021

As major structural EPS of the cystic fibrosis biofilm produced by mucoid Pseudomonas aeruginosa, alginate and its role in the tenacity of lung infection is a hot research topic.[1] It has been shown and proven that alginate plays a major role in shielding of antibiotics, such as aminoglycosides like Gentamycin [2] or Tobramycin (TS) [3]. However, to investigate in vitro the interaction between this EPS and drugs, the most reported models rely on either direct isolation of bacterial alginate or use native alginate derived from seaweed. As it is known that P. aeruginosa produces acetylated alginate, bacterial alginate seems more appropriate to use, but it comes with the drawback of tedious isolation processes and the need of extensive bacterial culturing [4]. One the other hand, native seaweed alginate, despite being commercially available, lacks acetylation and, hence, does not mimic CF-like biofilm environment. In this work we aim to investigate the influence of acetylation on the interaction between alginate and the aminoglycoside TS. Therefore, seaweed derived alginate was modified according to literature [5] and its interaction with TS was investigated at different scales. Bio-printed alginate microbeads were used to investigate macroscopic interaction, whereas interactions on a molecular level were assessed using isothermal titration calorimetry (ITC). Our results showed, for the first time, that the intensity of the interaction between alginate and TS highly depends on its degree of acetylation. Indeed, it was found, that acetylation strongly influences the shrinking behaviour of alginate beads once incubated in 0.1 wt% TS solution. We discovered also that not only the relative size after incubation but also the speed at which it shrinks were strongly affected by acetylation, as shown by Figure 1. First preliminary results in swelling and ITC experiments have proven that acetylation plays a key role in “shielding” drugs, like TS.

en

Cystic Fibrosis

Alginate acetylation

Tobramycin

The Influence of ACetylation on the Interachtion between Alginate and Tobramycin

Presentation

Vortrag

University of Natural Resources and Life Sciences, Vienna, Austria

University of Natural Resources and Life Sciences, Vienna, Austria

Poster Presentation

Analytical Instrumentation Center

M2

M1

M6

Materials Characterization

Surfaces and Interfaces

Biological and Bioactive Materials

20

20

60

E308-02-3 - Forschungsgruppe 3D Printing and Biofabrication

0000-0001-5064-2069

0000-0003-3112-5626

0000-0003-1417-5576

0000-0003-0735-113X

Break Biofilms Workshop 2023

FWF

P33226

16-01-2023

18-01-2023

On Site

Event for scientific audience

Wien

AT

Schandl, Stephan

Chemie

Pharmazie, Pharmakologie, Toxikologie

1040

3012

83

17

en

conference poster not in proceedings

none

no Fulltext

Publications

http://purl.org/coar/resource_type/c_18co

E308-02-3 - Forschungsgruppe 3D Printing and Biofabrication

BOKU University

BOKU University

E308-02-3 - Forschungsgruppe 3D Printing and Biofabrication

0000-0001-5064-2069

0000-0003-3112-5626

0000-0003-1417-5576

0000-0003-0735-113X

E308-02 - Forschungsbereich Polymer- und Verbundwerkstoffe

E308-02 - Forschungsbereich Polymer- und Verbundwerkstoffe