<div class="csl-bib-body">
<div class="csl-entry">Riedlsperger, L., Dabrowska, A., & Lendl, B. (2023, November 29). <i>QCL-based Multi-Pathlength Mid-Infrared Transmission Spectroscopy for Enhanced Analytical Capabilities</i> [Poster Presentation]. 18. Herbstkolloquium Prozessanalytik 2023, Krefeld, Germany. http://hdl.handle.net/20.500.12708/191092</div>
</div>
-
dc.identifier.uri
http://hdl.handle.net/20.500.12708/191092
-
dc.description.abstract
Mid-infrared spectroscopy, with its fixed transmission pathlength design, has long been a well-established method for the analysis of proteins and polypeptides in solution. Research shows that the optimum transmission of a solvent should be approximately 1/𝑒, resulting in an optimal absorbance of ~0.4 (or log10𝑒). [1] Achieving this value for a strong absorbing matrix proves challenging due to the varied background absorbance across different wavelengths. Proteins with their characteristic amide I (1700 – 1600 cm-1) and amide II (1600 – 1500 cm-1) bands are especially difficult to distinguish with spectroscopic analysis in aqueous solutions due to the strong water absorption in this region. In this work, we demonstrate a system that measures samples simultaneously at different pathlengths to determine the most optimal pathlength for each characteristic band of the solute. To achieve this, we employ an external-cavity quantum cascade laser (EC-QCL) that serves as a powerful source of IR radiation. The laser beam is subsequently expanded to a line and directed through the wedge-shaped transmission cell hosting pathlengths from ~10 μm to 400 μm. The transmitted radiation is then detected by a pyroelectric linear array with 256 elements. The hereby proposed configuration combines the advantages of quantum cascade lasers over conventional thermal emitters while also providing the capability to cover a wider dynamic range of absorbance values. We anticipate our setup to increase the signal-to-noise ratio for measurements of proteins in aqueous solutions and provide greater insight into sample and measurement results.
References:
[1] P. R. Griffiths, J. A. de Haseth, Fourier Transform Infrared Spectroscopy 2007, p. 256.
en
dc.language.iso
en
-
dc.subject
QCL/MIR Laser/Semiconductor Laser/Semiconductor
en
dc.subject
Multi-pathlength
en
dc.subject
Transmission Spectroscopy
en
dc.subject
Mid-IR spectroscopy
en
dc.title
QCL-based Multi-Pathlength Mid-Infrared Transmission Spectroscopy for Enhanced Analytical Capabilities
en
dc.type
Presentation
en
dc.type
Vortrag
de
dc.contributor.affiliation
TU Wien, Austria
-
dc.type.category
Poster Presentation
-
tuw.researchTopic.id
M2
-
tuw.researchTopic.id
Q1
-
tuw.researchTopic.name
Materials Characterization
-
tuw.researchTopic.name
Photonics
-
tuw.researchTopic.value
50
-
tuw.researchTopic.value
50
-
tuw.publication.orgunit
E164-02-1 - Forschungsgruppe Prozessanalytik
-
tuw.author.orcid
0009-0009-7577-4397
-
tuw.author.orcid
0000-0002-3074-5674
-
tuw.author.orcid
0000-0003-3838-5842
-
tuw.event.name
18. Herbstkolloquium Prozessanalytik 2023
en
dc.description.sponsorshipexternal
Horizon Europe
-
dc.relation.grantnoexternal
101119714
-
tuw.event.startdate
27-11-2023
-
tuw.event.enddate
29-11-2023
-
tuw.event.online
On Site
-
tuw.event.type
Event for scientific audience
-
tuw.event.place
Krefeld
-
tuw.event.country
DE
-
tuw.event.institution
Gesellschaft Deutscher Chemiker
-
tuw.event.presenter
Riedlsperger, Lisa
-
tuw.event.track
Single Track
-
wb.sciencebranch
Chemie
-
wb.sciencebranch.oefos
1040
-
wb.sciencebranch.value
100
-
item.openairecristype
http://purl.org/coar/resource_type/c_18co
-
item.openairetype
conference poster not in proceedings
-
item.fulltext
no Fulltext
-
item.languageiso639-1
en
-
item.grantfulltext
restricted
-
item.cerifentitytype
Publications
-
crisitem.author.dept
TU Wien
-
crisitem.author.dept
E164-02-1 - Forschungsgruppe Prozessanalytik
-
crisitem.author.dept
E164-02 - Forschungsbereich Umwelt-, Prozessanalytik und Sensoren
-
crisitem.author.orcid
0009-0009-7577-4397
-
crisitem.author.orcid
0000-0002-3074-5674
-
crisitem.author.orcid
0000-0003-3838-5842
-
crisitem.author.parentorg
E164-02 - Forschungsbereich Umwelt-, Prozessanalytik und Sensoren
-
crisitem.author.parentorg
E164 - Institut für Chemische Technologien und Analytik
