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<div class="csl-entry">Gonçalves, A., Leal, F., Moreira, A., Schellhorn, T., Blahnová, V. H., Zeiringer, S., Vocetková, K., Tetyczka, C., Simaite, A., Buzgo, M., Roblegg, E., Costa, P. F., Ertl, P., Filová, E., & Kohl, Y. (2023). Potential of Electrospun Fibrous Scaffolds for Intestinal, Skin, and Lung Epithelial Tissue Modeling. <i>Advanced NanoBiomed Research</i>, <i>3</i>(4), Article 2200104. https://doi.org/10.1002/anbr.202200104</div>
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/191679
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dc.description.abstract
Herein, intestinal, skin, and pulmonary in vitro tissue models based on electrospun membranes of poly(ε-caprolactone) (PCL) and cellulose acetate (CA), cellulose acetate phthalate (CAP), ethylcellulose (EC), or methylcellulose (MC) are presented. Physicochemical characterization and biocompatibility analyses of the scaffolds are carried out using colorectal adenocarcinoma cells (intestine), keratinocytes and fibroblasts (skin), and bronchial and alveolar epithelial cells (lung). PCL, PCL:CA, and PCL:EC are composed of nanofibers, whereas PCL:CAP and PCL:MC scaffolds comprise a combination of micro- and nanofibers. PCL, PCL:CA, PCL:CAP, and PCL:EC samples demonstrate water contact angles greater than 90° and are, therefore, hydrophobic, while PCL:MC mats display a hydrophilic behavior. In intestinal models, cells adhere and proliferate on all scaffolds; in turn, studies with skin cell models reveal that PCL:CA and PCL:CAP blends outperform all other substrates. Lung cell models show that, while 16HBE cells adhere to and proliferate in PCL, PCL:CA, PCL:EC, and PCL:MC scaffolds, A549 cells only have the same biological response on PCL, PCL:CA, and PCL:MC. In summary, all fibrous meshes prepared are biocompatible toward most cell types tested, thus suggesting the potential of PCL-cellulose derivative blends as substrates suitable for in vitro epithelial tissue modeling and toxicity screening.
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dc.language.iso
en
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dc.publisher
Wiley-VCH
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dc.relation.ispartof
Advanced NanoBiomed Research
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dc.subject
3R principle
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dc.subject
cellulose
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dc.subject
electrospinning
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dc.subject
epithelial barrier
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dc.subject
in vitro models
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dc.subject
toxicological screening
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dc.title
Potential of Electrospun Fibrous Scaffolds for Intestinal, Skin, and Lung Epithelial Tissue Modeling