<div class="csl-bib-body">
<div class="csl-entry">Kopinski-Grünwald, O., Guillaume, O., Ferner, T., Schädl, B., & Ovsianikov, A. (2024). Scaffolded spheroids as building blocks for bottom-up cartilage tissue engineering show enhanced bioassembly dynamics. <i>Acta Biomaterialia</i>, <i>174</i>, 163–176. https://doi.org/10.1016/j.actbio.2023.12.001</div>
</div>
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dc.identifier.issn
1742-7061
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/191740
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dc.description.abstract
Due to the capability of cell spheroids (SPH) to assemble into large high cell density constructs, their use as building blocks attracted a lot of attention in the field of biofabrication. Nevertheless, upon maturation, the composition along with the size of such building blocks change, affecting their fusiogenic ability to form a cohesive tissue construct of controllable size. This natural phenomenon remains a limitation for the standardization of spheroid-based therapies in the clinical setting.
We recently showed that scaffolded spheroids (S-SPH) can be produced by forming spheroids directly within porous PCL-based microscaffolds fabricated using multiphoton lithography (MPL). In this new study, we compare the bioassembly potential of conventional SPHs versus S-SPHs depending on their degree of maturation. Doublets of both types of building blocks were cultured and their fusiogenicity was compared by measuring the intersphere angle, the length of the fusing spheroid pairs (referred to as doublet length) as well as their spreading behaviour. Finally, the possibility to fabricate macro-sized tissue constructs (i.e. cartilage-like) from both chondrogenic S-SPHs and SPHs was analyzed.
This study revealed that, in contrast to conventional SPHs, S-SPHs exhibit robust and stable fusiogenicity, independently from their degree of maturation. In order to understand this behavior, we further analyze the intersection area of doublets, looking at the kinetic of cell migration and at the mechanical stability of the formed tissue using dissection measurements. Our findings indicate that the presence of microscaffolds enhances the ability of spheroids to be used as building blocks for bottom-up tissue engineering, which is an important advantage compared to conventional spheroid-based therapy approaches.
en
dc.description.sponsorship
European Commission
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dc.language.iso
en
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dc.publisher
ELSEVIER SCI LTD
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dc.relation.ispartof
Acta Biomaterialia
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dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
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dc.subject
Bioassembly
en
dc.subject
Cartilage
en
dc.subject
Scaffolded spheroids
en
dc.subject
Spheroids
en
dc.subject
tissue engineering
en
dc.title
Scaffolded spheroids as building blocks for bottom-up cartilage tissue engineering show enhanced bioassembly dynamics
en
dc.type
Article
en
dc.type
Artikel
de
dc.rights.license
Creative Commons Namensnennung 4.0 International
de
dc.rights.license
Creative Commons Attribution 4.0 International
en
dc.identifier.pmid
38065247
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dc.contributor.affiliation
Austrian Cluster for Tissue Regeneration, Austria; Ludwig Boltzmann Institute for Experimental and Clinical Traumatology in AUVA Trauma Research Center, Austria; Medical University of Vienna, Austria
Third Strategy in Tissue Engineering ¿ Functional microfabricated multicellular spheroid carriers for tissue engineering and regeneration
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tuw.researchTopic.id
M6
-
tuw.researchTopic.id
X1
-
tuw.researchTopic.name
Biological and Bioactive Materials
-
tuw.researchTopic.name
Beyond TUW-research foci
-
tuw.researchTopic.value
80
-
tuw.researchTopic.value
20
-
dcterms.isPartOf.title
Acta Biomaterialia
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tuw.publication.orgunit
E308-02-3 - Forschungsgruppe 3D Printing and Biofabrication
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tuw.publisher.doi
10.1016/j.actbio.2023.12.001
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dc.date.onlinefirst
2023-12-07
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dc.identifier.eissn
1878-7568
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dc.identifier.libraryid
AC17204936
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dc.description.numberOfPages
14
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tuw.author.orcid
0000-0003-1842-7697
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tuw.author.orcid
0000-0003-0735-113X
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tuw.author.orcid
0000-0002-6712-6279
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tuw.author.orcid
0000-0001-5846-0198
-
dc.rights.identifier
CC BY 4.0
de
dc.rights.identifier
CC BY 4.0
en
wb.sci
true
-
wb.sciencebranch
Maschinenbau
-
wb.sciencebranch
Werkstofftechnik
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wb.sciencebranch.oefos
2030
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wb.sciencebranch.oefos
2050
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wb.sciencebranch.value
20
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wb.sciencebranch.value
80
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item.openaccessfulltext
Open Access
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item.cerifentitytype
Publications
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item.languageiso639-1
en
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item.openairecristype
http://purl.org/coar/resource_type/c_2df8fbb1
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item.grantfulltext
open
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item.fulltext
with Fulltext
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item.mimetype
application/pdf
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item.openairetype
research article
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crisitem.author.dept
E308-02-3 - Forschungsgruppe 3D Printing and Biofabrication
-
crisitem.author.dept
E308-02-3 - Forschungsgruppe 3D Printing and Biofabrication
-
crisitem.author.dept
TU Wien
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crisitem.author.dept
Austrian Cluster for Tissue Regeneration, Austria; Ludwig Boltzmann Institute for Experimental and Clinical Traumatology in AUVA Trauma Research Center, Austria; Medical University of Vienna, Austria
-
crisitem.author.dept
E308-02-3 - Forschungsgruppe 3D Printing and Biofabrication
-
crisitem.author.orcid
0000-0003-1842-7697
-
crisitem.author.orcid
0000-0003-0735-113X
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crisitem.author.orcid
0000-0002-6712-6279
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crisitem.author.orcid
0000-0001-5846-0198
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crisitem.author.parentorg
E308-02 - Forschungsbereich Polymer- und Verbundwerkstoffe
-
crisitem.author.parentorg
E308-02 - Forschungsbereich Polymer- und Verbundwerkstoffe
-
crisitem.author.parentorg
E308-02 - Forschungsbereich Polymer- und Verbundwerkstoffe