<div class="csl-bib-body">
<div class="csl-entry">Oleinik, E., Teuschl-Woller, A., Weihs, A. M., & Thurner, P. (2023, May 23). <i>The effect of scaffold stiffness on cellular alignment in tendon tissue engineering</i> [Conference Presentation]. ESB Austrian Chapter meeting, 2023, Salzburg, Austria.</div>
</div>
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/192467
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dc.description.abstract
A universal and approved in vitro tendon model that closely resembles the physiological characteristics of tendons currently is needed. Such models can be used to study different pathologies such as tendinopathy. Tendon models usually rely on mechanical stimulation, which requires the determination of stimulation parameters alongside scaffold selection, characterization and optimization, to induce cellular alignment and production of proper ECM.
Here, we employed a tension-based MagneTissue bioreactor to investigate the effect of substrate stiffness on cell morphology. Briefly, 3D fibrin rings were seeded with C3H10T1/2 and C2C12 cells. Cells were cultivated in growth medium and static mechanical strain was subsequently applied. Cell viability and cell alignment were analyzed on day 7 using CalceinAM/PI staining.
Cells in both cell lines were viable and had a homogeneous distribution throughout the scaffold. However, the morphology of cells varied. Loaded C2C12 cells were more aligned along strain direction compared to gravity control. In comparison, no differences in cell alignment were observed between loaded and control groups in the C3H10T1/2 cell line. This suggests that scaffold stiffness is not favorable for C3H10T1/2 cells and should be optimized for each cell type to limit cell death and facilitate cell adhesion, alignment, and differentiation
en
dc.description.sponsorship
FWF - Österr. Wissenschaftsfonds
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dc.language.iso
en
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dc.subject
tendon
en
dc.subject
tissue engineering
en
dc.subject
fibrin
en
dc.title
The effect of scaffold stiffness on cellular alignment in tendon tissue engineering
en
dc.type
Presentation
en
dc.type
Vortrag
de
dc.contributor.affiliation
University of Applied Sciences Technikum Wien, Vienna, Austria; The Austrian Cluster for Tissue Regeneration, Vienna, Austria
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dc.contributor.affiliation
University of Applied Sciences Technikum Wien, Vienna, Austria; The Austrian Cluster for Tissue Regeneration, Vienna, Austria
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dc.relation.grantno
DFH 28
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dc.type.category
Conference Presentation
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tuw.project.title
Erzeugung funktioneller Gewebe mit Hilfe von Bioreaktoren und Mikrofluidik.
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tuw.researchTopic.id
M6
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tuw.researchTopic.id
C6
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tuw.researchTopic.name
Biological and Bioactive Materials
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tuw.researchTopic.name
Modeling and Simulation
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tuw.researchTopic.value
50
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tuw.researchTopic.value
50
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tuw.publication.orgunit
E317-02 - Forschungsbereich Biomechanik
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tuw.author.orcid
0000-0002-3817-1572
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tuw.author.orcid
0000-0001-7588-9041
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tuw.event.name
ESB Austrian Chapter meeting, 2023
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tuw.event.startdate
23-05-2023
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tuw.event.enddate
23-05-2023
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tuw.event.online
On Site
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tuw.event.type
Event for scientific audience
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tuw.event.place
Salzburg
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tuw.event.country
AT
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tuw.event.institution
PMU, Salzburg
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tuw.event.presenter
Oleinik, Ekaterina
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wb.sciencebranch
Maschinenbau
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wb.sciencebranch
Sonstige Technische Wissenschaften
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wb.sciencebranch
Sonstige Humanmedizin, Gesundheitswissenschaften
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wb.sciencebranch.oefos
2030
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wb.sciencebranch.oefos
2119
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wb.sciencebranch.oefos
3059
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wb.sciencebranch.value
40
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wb.sciencebranch.value
30
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wb.sciencebranch.value
30
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item.cerifentitytype
Publications
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item.fulltext
no Fulltext
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item.languageiso639-1
en
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item.openairetype
conference paper not in proceedings
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item.grantfulltext
restricted
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item.openairecristype
http://purl.org/coar/resource_type/c_18cp
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crisitem.author.dept
E317-02 - Forschungsbereich Biomechanik
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crisitem.author.dept
University of Applied Sciences Technikum Wien, Vienna, Austria; The Austrian Cluster for Tissue Regeneration, Vienna, Austria
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crisitem.author.dept
University of Applied Sciences Technikum Wien, Vienna, Austria; The Austrian Cluster for Tissue Regeneration, Vienna, Austria
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crisitem.author.dept
E317 - Institut für Leichtbau und Struktur-Biomechanik
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crisitem.author.orcid
0000-0002-3817-1572
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crisitem.author.orcid
0000-0001-7588-9041
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crisitem.author.parentorg
E317 - Institut für Leichtbau und Struktur-Biomechanik
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crisitem.author.parentorg
E300 - Fakultät für Maschinenwesen und Betriebswissenschaften