<div class="csl-bib-body">
<div class="csl-entry">Vieira Dias Dos Santos, A. C., Hondl, N., Ramos Garcia, M., Victoria, Kuligowski, J., Lendl, B., & Ramer, G. (2023). AFM-IR for nanoscale chemical characterization in life sciences: recent developments and future directions. <i>ACS Measurement Science Au</i>, <i>3</i>(5), 301–314. https://doi.org/10.1021/acsmeasuresciau.3c00010</div>
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/192537
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dc.description.abstract
Despite the ubiquitous absorption of mid-infrared (IR) radiation by virtually all molecules that belong to the major biomolecules groups (proteins, lipids, carbohydrates, nucleic acids), the application of conventional IR microscopy to the life sciences remained somewhat limited, due to the restrictions on spatial resolution imposed by the diffraction limit (in the order of several micrometers). This issue is addressed by AFM-IR, a scanning probe-based technique that allows for chemical analysis at the nanoscale with resolutions down to 10 nm and thus has the potential to contribute to the investigation of nano and microscale biological processes. In this perspective, in addition to a concise description of the working principles and operating modes of AFM-IR, we present and evaluate the latest key applications of AFM-IR to the life sciences, summarizing what the technique has to offer to this field. Furthermore, we discuss the most relevant current limitations and point out potential future developments and areas for further application for fruitful interdisciplinary collaboration.
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dc.description.sponsorship
FFG - Österr. Forschungsförderungs- gesellschaft mbH
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dc.description.sponsorship
European Commission
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dc.description.sponsorship
European Commission
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dc.language.iso
en
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dc.publisher
American Chemical Society (ACS)
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dc.relation.ispartof
ACS Measurement Science Au
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dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
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dc.subject
Absorption
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dc.subject
Infrared light
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dc.subject
Lasers
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dc.subject
Lipids
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dc.subject
Liquids
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dc.title
AFM-IR for nanoscale chemical characterization in life sciences: recent developments and future directions