<div class="csl-bib-body">
<div class="csl-entry">Hermann, D.-R., Ramer, G., Riedlsperger, L., & Lendl, B. (2023). Chiral sensing for liquid analysis – Synergies between Quantum Cascade Lasers and Vibrational Circular Dichroism. In <i>ICAVS12: 12th International Conference on Advanced Vibrational Spectroscopy: Abstract Book</i> (pp. 344–344). Faculty of Chemistry Jagiellonian University in Krakow.</div>
</div>
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/192774
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dc.description.abstract
Both chiral small molecules and protein derived pharmaceuticals like monoclonal antibodies have been the focus of increased development in the last years, resulting in an increased need for analytics.1,2 For both categories, their therapeutic effect is intrinsically linked to their structure and indeed their chirality. It is therefore desirable to monitor and control these parameters, the enantiomeric excess for small molecules and the secondary and tertiary structure for proteins, over the production cycle of a drug. Vibrational
circular dichroism (VCD) can be used in this regard, as it expands the inherent structure elucidation capabilities of mid-infrared (MIR) spectroscopy by adding chiral sensitivity as additional dimension. Unfortunately, the application of VCD is limited by several factors.
The high absorbance of some solvents, like water during protein analytics, restricts the pathlength and therefore the achievable intensity, resulting in high limits of detection. Additionally, this drawback is compounded by VCD being characterized by very low intensity (~10-5 compared to classical absorbance), necessitating long acquisition times (hours) to achieve acceptable noise levels. We now present a dedicated VCD setup, utilizing a quantum cascade laser (QCL), as a viable solution to the discussed challenges. With the high brilliance of the QCL longer pathlengths, resulting in stronger signals, are possible. Our implementation of balanced detection for VCD measurements achieves a considerable reduction of laser noise, providing further advantages.
We employed these advances for high time resolution studies of small molecules at a time scale of 3 minutes per measurement and low concentration measurements of the amide I band of proteins in water at 18 minutes of spectral acquisition.
en
dc.description.sponsorship
FFG - Österr. Forschungsförderungs- gesellschaft mbH
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dc.language.iso
en
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dc.subject
Quantum cascade laser
en
dc.subject
Vibrational circular dichroism
en
dc.title
Chiral sensing for liquid analysis – Synergies between Quantum Cascade Lasers and Vibrational Circular Dichroism
en
dc.type
Inproceedings
en
dc.type
Konferenzbeitrag
de
dc.contributor.affiliation
TU Wien, Österreich
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dc.description.startpage
344
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dc.description.endpage
344
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dc.relation.grantno
868615
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dc.type.category
Abstract Book Contribution
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tuw.booktitle
ICAVS12: 12th International Conference on Advanced Vibrational Spectroscopy: Abstract Book
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tuw.relation.publisher
Faculty of Chemistry Jagiellonian University in Krakow
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tuw.relation.publisherplace
Krakau
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tuw.project.title
Chemical Systems Engineering K1 Centre
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tuw.researchTopic.id
Q1
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tuw.researchTopic.name
Photonics
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tuw.researchTopic.value
100
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tuw.publication.orgunit
E164-02-1 - Forschungsgruppe Prozessanalytik
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dc.description.numberOfPages
1
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tuw.author.orcid
000-0001-8307-5435
-
tuw.author.orcid
0009-0009-7577-4397
-
tuw.author.orcid
0000-0003-3838-5842
-
tuw.event.name
12th International Conference on Advanced Vibrational Spectroscopy (ICAVS 12) 2023
en
tuw.event.startdate
27-08-2023
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tuw.event.enddate
01-09-2023
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tuw.event.online
On Site
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tuw.event.type
Event for scientific audience
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tuw.event.place
Krakau
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tuw.event.country
PL
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tuw.event.institution
Faculty of Chemistry Jagiellonian University in Krakow
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tuw.event.presenter
Hermann, Daniel-Ralph
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tuw.event.track
Multi Track
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wb.sciencebranch
Chemie
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wb.sciencebranch
Physik, Astronomie
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wb.sciencebranch.oefos
1040
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wb.sciencebranch.oefos
1030
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wb.sciencebranch.value
60
-
wb.sciencebranch.value
40
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item.languageiso639-1
en
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item.openairecristype
http://purl.org/coar/resource_type/c_5794
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item.openairetype
conference paper
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item.cerifentitytype
Publications
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item.fulltext
no Fulltext
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item.grantfulltext
none
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crisitem.author.dept
E164-02-1 - Forschungsgruppe Prozessanalytik
-
crisitem.author.dept
E164-02-1 - Forschungsgruppe Prozessanalytik
-
crisitem.author.dept
TU Wien, Österreich
-
crisitem.author.dept
E164-02 - Forschungsbereich Umwelt-, Prozessanalytik und Sensoren
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crisitem.author.orcid
0000-0001-8307-5435
-
crisitem.author.orcid
0009-0009-7577-4397
-
crisitem.author.orcid
0000-0003-3838-5842
-
crisitem.author.parentorg
E164-02 - Forschungsbereich Umwelt-, Prozessanalytik und Sensoren
-
crisitem.author.parentorg
E164-02 - Forschungsbereich Umwelt-, Prozessanalytik und Sensoren
-
crisitem.author.parentorg
E164 - Institut für Chemische Technologien und Analytik
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crisitem.project.funder
FFG - Österr. Forschungsförderungs- gesellschaft mbH