<div class="csl-bib-body">
<div class="csl-entry">Iebed, D., & Conibear, A. C. (2023, December 5). <i>Elucidating the role of posttranslational modifications of HMGN1 in DNA packaging</i> [Poster Presentation]. Austrian Peptide Symposium, Austria.</div>
</div>
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/193045
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dc.description.abstract
DNA packaging in eukaryotes is a tightly regulated mechanism involving a number of different proteins. Among them is HMGN1, a member of the intrinsically disordered high mobility group proteins. HMGN1 is modulated by its posttranslational modifications (PTMs), mainly lysine acetylations and serine phosphorylations. Understanding the specific influence of PTMs on the biological function of HMGN1 is a challenge, because of the difficulties of accessing homogeneous site-specifically modified HMGN1 variants. Here we present our synthetic approach to accessing HMGN1 variants bearing site-specific fluorophores, and PTMs in the nucleosome binding domain and nuclear localization signal. We investigated several semi-synthetic strategies and native chemical ligation sites for their feasibility, synthetic accessibility and efficiency. The site-specifically modified HMGN1 variants will be used to investigate the effect of the PTMs on the binding of HMGN1 to nucleosomes, the localization of HMGN1 in the cell, and conformational changes. PTM crosstalk, in which the effects of multiple PTMs interact, will be explored as well. Understanding how PTMs influence the behavior of HMGN1 in DNA packaging has the potential to provide insights into how this fundamental cellular process is regulated.
en
dc.description.sponsorship
FWF - Österr. Wissenschaftsfonds
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dc.language.iso
en
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dc.subject
protein semi-synthesis
en
dc.subject
posttranlational modifications
en
dc.subject
Intrinsically disordered proteins
en
dc.subject
protein NMR spectroscopy
en
dc.title
Elucidating the role of posttranslational modifications of HMGN1 in DNA packaging
en
dc.type
Presentation
en
dc.type
Vortrag
de
dc.relation.grantno
P 36101-B
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dc.type.category
Poster Presentation
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tuw.project.title
Posttranslationale Modifikation von HMGN1 in DNA-Verpackung
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tuw.researchinfrastructure
Zentrum für Kernspinresonanzspektroskopie
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tuw.researchTopic.id
M6
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tuw.researchTopic.id
M8
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tuw.researchTopic.name
Biological and Bioactive Materials
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tuw.researchTopic.name
Structure-Property Relationsship
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tuw.researchTopic.value
85
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tuw.researchTopic.value
15
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tuw.publication.orgunit
E163-03-2 - Forschungsgruppe Molekulare Chemie und Chemische Biologie
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tuw.author.orcid
0009-0008-7920-1206
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tuw.author.orcid
0000-0002-5482-6225
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tuw.event.name
Austrian Peptide Symposium
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tuw.event.startdate
05-12-2023
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tuw.event.enddate
05-12-2023
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tuw.event.online
On Site
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tuw.event.type
Event for scientific audience
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tuw.event.country
AT
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tuw.event.presenter
Iebed, Dina
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wb.sciencebranch
Chemie
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wb.sciencebranch
Chemische Verfahrenstechnik
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wb.sciencebranch
Pharmazie, Pharmakologie, Toxikologie
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wb.sciencebranch.oefos
1040
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wb.sciencebranch.oefos
2040
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wb.sciencebranch.oefos
3012
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wb.sciencebranch.value
60
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wb.sciencebranch.value
20
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wb.sciencebranch.value
20
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item.languageiso639-1
en
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item.openairetype
conference poster not in proceedings
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item.grantfulltext
restricted
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item.fulltext
no Fulltext
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item.cerifentitytype
Publications
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item.openairecristype
http://purl.org/coar/resource_type/c_18co
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crisitem.author.dept
E163-03-2 - Forschungsgruppe Molekulare Chemie und Chemische Biologie
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crisitem.author.dept
E163-03-2 - Forschungsgruppe Molekulare Chemie und Chemische Biologie
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crisitem.author.orcid
0009-0008-7920-1206
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crisitem.author.orcid
0000-0002-5482-6225
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crisitem.author.parentorg
E163-03 - Forschungsbereich Organische und Biologische Chemie
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crisitem.author.parentorg
E163-03 - Forschungsbereich Organische und Biologische Chemie
