<div class="csl-bib-body">
<div class="csl-entry">Salvadori, A., Watanabe, M., Markovic, M., & Ovsianikov, A. (2023, November 2). <i>Laser-Generated Vascular Structures for Liver-on-a-Chip</i> [Poster Presentation]. LBG Meeting Innovation in Health Sciences 2023, Wien, Austria.</div>
</div>
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/193313
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dc.description.abstract
Organs-on-Chips (OoC) consist of multicellular constructs organized in a 3D manner to faithfully replicate in vitro specific functions of human organs or tissues in a highly controlled microenvironment. However, the lack of a proper and functional microvascular network remains a significant challenge, which prevents a steady supply of nutrients and oxygen and, therefore, long-term maintenance in terms of viability, morphology, and functionality. Various biofabrication methods, such as 3D bioprinting and templating strategies, have been implemented to guide and stimulate the formation of microvascular networks in a controlled and reproducible way but limited in resolution and precision (1,2). Here, we demonstrate how femtosecond laser-based bioprinting techniques could guide the endothelial cells growth and lead to perfusable and physiologically relevant microvascular structures directly on-chip.
A microfluidic device was loaded with a mixture of type I-collagen:Matrigel (3:1) prepared following a two-step gelation protocol and two-photon laser patterning was performed across the hydrogel region to create hollow cylindrical channels. HUVECs (Lonza) were seeded in both side channels, and a VEGF gradient was established across the hydrogel to stimulate their controlled proliferation and migration. At seven days, the vascular structures were perfused with FITC-Dextran to study the barrier function and stained for specific endothelial key markers, such as CD31, VE-cadherin, and ZO-1.
We demonstrated that the two-photon laser patterning led to forming perfusable cylindrical channels, faithful in size and geometry. In addition, the two-step gelation protocol minimized the cell-induced channels’ deformation, creating fully-endothelialized vascular structures stable for at least seven days of culture. High expression of CD31, VE-cadherin, and ZO-1 confirmed the presence of adherent junctions and intercellular connections. Moreover, the diffusion of FITC-Dextran demonstrated a reduced diffusional permeability in the abluminal region compared to the acellular channels, suggesting the reproduction of vascular barrier function. Future works will focus on replicating organ-specific microvasculature for OoCs in terms of viability, morphology, and functionality maintenance.
en
dc.language.iso
en
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dc.subject
Two-photon patterning
en
dc.subject
Microvasculature
en
dc.subject
Organ-on-chip
en
dc.subject
Channels' endothelialization
en
dc.subject
Endothelial barrier function
en
dc.title
Laser-Generated Vascular Structures for Liver-on-a-Chip
en
dc.type
Presentation
en
dc.type
Vortrag
de
dc.type.category
Poster Presentation
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tuw.researchinfrastructure
Analytical Instrumentation Center
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tuw.researchTopic.id
M6
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tuw.researchTopic.name
Biological and Bioactive Materials
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tuw.researchTopic.value
100
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tuw.publication.orgunit
E308-02-3 - Forschungsgruppe 3D Printing and Biofabrication
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tuw.author.orcid
0000-0001-8984-5672
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tuw.author.orcid
0000-0001-5846-0198
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tuw.event.name
LBG Meeting Innovation in Health Sciences 2023
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tuw.event.startdate
02-11-2023
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tuw.event.enddate
03-11-2023
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tuw.event.online
On Site
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tuw.event.type
Event for scientific audience
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tuw.event.place
Wien
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tuw.event.country
AT
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tuw.event.institution
Ludwig Boltzmann Gessellschaft
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tuw.event.presenter
Salvadori, Alice
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wb.sciencebranch
Maschinenbau
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wb.sciencebranch
Werkstofftechnik
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wb.sciencebranch.oefos
2030
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wb.sciencebranch.oefos
2050
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wb.sciencebranch.value
20
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wb.sciencebranch.value
80
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item.fulltext
no Fulltext
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item.grantfulltext
none
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item.languageiso639-1
en
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item.openairetype
conference poster not in proceedings
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item.cerifentitytype
Publications
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item.openairecristype
http://purl.org/coar/resource_type/c_18co
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crisitem.author.dept
E308-02-3 - Forschungsgruppe 3D Printing and Biofabrication
-
crisitem.author.dept
E308-02-3 - Forschungsgruppe 3D Printing and Biofabrication
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crisitem.author.dept
E308-50-2 - Fachgruppe Technische Assistenz
-
crisitem.author.dept
E308-02-3 - Forschungsgruppe 3D Printing and Biofabrication
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crisitem.author.orcid
0000-0001-8984-5672
-
crisitem.author.orcid
0000-0001-5846-0198
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crisitem.author.parentorg
E308-02 - Forschungsbereich Polymer- und Verbundwerkstoffe
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crisitem.author.parentorg
E308-02 - Forschungsbereich Polymer- und Verbundwerkstoffe
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crisitem.author.parentorg
E308-50 - Services des Instituts
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crisitem.author.parentorg
E308-02 - Forschungsbereich Polymer- und Verbundwerkstoffe