Oleinik, Ekaterina

Teuschl-Woller, Andreas

Weihs, Anna M.

Külekci, Büşra

Thurner, Philipp

2024-02-06T14:09:54Z

2024-02-06T14:09:54Z

2023-12-12

<div class="csl-bib-body"> <div class="csl-entry">Oleinik, E., Teuschl-Woller, A., Weihs, A. M., Külekci, B., &#38; Thurner, P. (2023, December 12). <i>Effect of scaffold stiffness and mechanical stimulation on tendon tissue engineered constructs</i> [Conference Presentation]. ViCEM-Vienna Center for Engineering in Medicine 2023, Austria. http://hdl.handle.net/20.500.12708/193532</div> </div>

http://hdl.handle.net/20.500.12708/193532

Tendinopathies are becoming more prevalent, yet their exact cause is not always clear. It is hypothesized that inflammation plays a crucial role in extracellular matrix remodeling and therefore, compromises the mechanical and biological characteristics of tendons. The lack of a tendon tissue model makes it difficult to verify this hypothesis. A universal and approved in vitro tendon model that closely resembles the physiological characteristics of tendons currently is needed. Tendon models usually rely on mechanical stimulation, which requires the determination of stimulation parameters alongside scaffold selection, characterization, and optimization, to induce cellular alignment and production of proper ECM. Here, we employed a tension-based MagneTissue bioreactor to establish a tendon-like tissue model by controlled mechanical stimulation of tendon-derived progenitor cells (TDPCs) embedded in fibrin constructs. Briefly, 3D fibrin rings were seeded with TDPCs. Cells were cultivated in a growth medium and mechanical strain in two different ways (static load and cyclic load) was subsequently applied. Cell viability, and alignment as well as their localization and density within the construct were analyzed 7 days after stimulation using immunofluorescent staining. Cells in both cell lines were viable and aligned towards the axis of strain. However, the distribution of cells throughout the scaffold varied. In both groups, cells were predominantly located on the surface and there was no difference in cell number. Although, there was a tendency to have more cells inside in the group of cyclic load compared to both the static load and non-loaded groups. Additionally, cyclic-loaded samples showed fewer fragmented nuclei inside the construct, which might indicate a lower level of apoptosis. We can assume that cyclic loading appears to improve media penetration and cell survival and it is a crucial stimulation parameter to generate tendon-like constructs.

FWF - Österr. Wissenschaftsfonds

en

tendon

apoptosis

fibrin

mechanical stimulation

tissue engineering

Effect of scaffold stiffness and mechanical stimulation on tendon tissue engineered constructs

Presentation

Vortrag

University of Applied Sciences Technikum Wien, Austria

University of Applied Sciences Technikum Wien, Austria

Medical University of Vienna, Austria

DFH 28

Conference Presentation

Erzeugung funktioneller Gewebe mit Hilfe von Bioreaktoren und Mikrofluidik.

M6

C6

Biological and Bioactive Materials

Modeling and Simulation

50

50

E317-02 - Forschungsbereich Biomechanik

0000-0002-3817-1572

0000-0002-4074-6021

0000-0001-7588-9041

ViCEM-Vienna Center for Engineering in Medicine 2023

12-12-2023

13-12-2023

On Site

Event for scientific audience

AT

MedUniWien

Oleinik, Ekaterina

Maschinenbau

Biologie

Andere Naturwissenschaften

2030

1060

1070

40

30

30

no Fulltext

en

Publications

none

http://purl.org/coar/resource_type/c_18cp

conference paper not in proceedings

FWF - Österr. Wissenschaftsfonds

DFH 28

E317-02 - Forschungsbereich Biomechanik

University of Applied Sciences Technikum Wien, Austria

University of Applied Sciences Technikum Wien, Austria

Medical University of Vienna, Austria

E317 - Institut für Leichtbau und Struktur-Biomechanik

0000-0002-3817-1572

0000-0002-4074-6021

0000-0001-7588-9041

E317 - Institut für Leichtbau und Struktur-Biomechanik

E300 - Fakultät für Maschinenwesen und Betriebswissenschaften