<div class="csl-bib-body">
<div class="csl-entry">Oleinik, E., Teuschl-Woller, A., Weihs, A. M., Külekci, B., & Thurner, P. (2023, December 12). <i>Effect of scaffold stiffness and mechanical stimulation on tendon tissue engineered constructs</i> [Conference Presentation]. ViCEM-Vienna Center for Engineering in Medicine 2023, Austria.</div>
</div>
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/193532
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dc.description.abstract
Tendinopathies are becoming more prevalent, yet their exact cause is not always clear. It is hypothesized that inflammation plays a crucial role in extracellular matrix remodeling and therefore, compromises the mechanical and biological characteristics of tendons. The lack of a tendon tissue model makes it difficult to verify this hypothesis. A universal and approved in vitro tendon model that closely resembles the physiological characteristics of tendons currently is needed. Tendon models usually rely on mechanical stimulation, which requires the determination of stimulation parameters alongside scaffold selection, characterization, and optimization, to induce cellular alignment and production of proper ECM.
Here, we employed a tension-based MagneTissue bioreactor to establish a tendon-like tissue model by controlled mechanical stimulation of tendon-derived progenitor cells (TDPCs) embedded in fibrin constructs. Briefly, 3D fibrin rings were seeded with TDPCs. Cells were cultivated in a growth medium and mechanical strain in two different ways (static load and cyclic load) was subsequently applied. Cell viability, and alignment as well as their localization and density within the construct were analyzed 7 days after stimulation using immunofluorescent staining.
Cells in both cell lines were viable and aligned towards the axis of strain. However, the distribution of cells throughout the scaffold varied. In both groups, cells were predominantly located on the surface and there was no difference in cell number. Although, there was a tendency to have more cells inside in the group of cyclic load compared to both the static load and non-loaded groups. Additionally, cyclic-loaded samples showed fewer fragmented nuclei inside the construct, which might indicate a lower level of apoptosis. We can assume that cyclic loading appears to improve media penetration and cell survival and it is a crucial stimulation parameter to generate tendon-like constructs.
en
dc.description.sponsorship
FWF - Österr. Wissenschaftsfonds
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dc.language.iso
en
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dc.subject
tendon
en
dc.subject
apoptosis
en
dc.subject
fibrin
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dc.subject
mechanical stimulation
en
dc.subject
tissue engineering
en
dc.title
Effect of scaffold stiffness and mechanical stimulation on tendon tissue engineered constructs
en
dc.title.alternative
Effect of cyclic loading on survival of cells in tissue-engineered tendon constructs
en
dc.type
Presentation
en
dc.type
Vortrag
de
dc.contributor.affiliation
University of Applied Sciences Technikum Wien, Austria
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dc.contributor.affiliation
University of Applied Sciences Technikum Wien, Vienna, Austria; The Austrian Cluster for Tissue Regeneration, Vienna, Austria
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dc.contributor.affiliation
Medical University of Vienna
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dc.relation.grantno
DFH 28
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dc.type.category
Conference Presentation
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tuw.project.title
Erzeugung funktioneller Gewebe mit Hilfe von Bioreaktoren und Mikrofluidik.
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tuw.researchTopic.id
M6
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tuw.researchTopic.id
C6
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tuw.researchTopic.name
Biological and Bioactive Materials
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tuw.researchTopic.name
Modeling and Simulation
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tuw.researchTopic.value
50
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tuw.researchTopic.value
50
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tuw.publication.orgunit
E317-02 - Forschungsbereich Biomechanik
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tuw.author.orcid
0000-0002-3817-1572
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tuw.author.orcid
0000-0002-4074-6021
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tuw.author.orcid
0000-0001-7588-9041
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tuw.event.name
ViCEM-Vienna Center for Engineering in Medicine 2023
en
tuw.event.startdate
12-12-2023
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tuw.event.enddate
13-12-2023
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tuw.event.online
On Site
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tuw.event.type
Event for scientific audience
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tuw.event.country
AT
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tuw.event.institution
MedUniWien
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tuw.event.presenter
Oleinik, Ekaterina
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wb.sciencebranch
Maschinenbau
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wb.sciencebranch
Biologie
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wb.sciencebranch
Andere Naturwissenschaften
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wb.sciencebranch.oefos
2030
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wb.sciencebranch.oefos
1060
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wb.sciencebranch.oefos
1070
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wb.sciencebranch.value
40
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wb.sciencebranch.value
30
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wb.sciencebranch.value
30
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item.openairecristype
http://purl.org/coar/resource_type/c_18cp
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item.cerifentitytype
Publications
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item.grantfulltext
none
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item.fulltext
no Fulltext
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item.languageiso639-1
en
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item.openairetype
conference paper not in proceedings
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crisitem.project.funder
FWF - Österr. Wissenschaftsfonds
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crisitem.project.grantno
DFH 28
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crisitem.author.dept
E317-02 - Forschungsbereich Biomechanik
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crisitem.author.dept
University of Applied Sciences Technikum Wien, Austria
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crisitem.author.dept
University of Applied Sciences Technikum Wien, Vienna, Austria; The Austrian Cluster for Tissue Regeneration, Vienna, Austria
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crisitem.author.dept
Medical University of Vienna
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crisitem.author.dept
E317 - Institut für Leichtbau und Struktur-Biomechanik
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crisitem.author.orcid
0000-0002-3817-1572
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crisitem.author.orcid
0000-0002-4074-6021
-
crisitem.author.orcid
0000-0001-7588-9041
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crisitem.author.parentorg
E317 - Institut für Leichtbau und Struktur-Biomechanik
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crisitem.author.parentorg
E300 - Fakultät für Maschinenwesen und Betriebswissenschaften