<div class="csl-bib-body">
<div class="csl-entry">Schmal, M. (2023, March 8). <i>Phylogenetic analysis and investigation of RiPP cluster in 50 Trichoderma genomes</i> [Conference Presentation]. 16th European Conference on Fungal Genetics (ECFG16), Innsbruck, Austria.</div>
</div>
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/193538
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dc.description.abstract
Many Trichoderma species have applications in biotechnology, agriculture and human healthcare. Often, secondary metabolites play an important role in the biological activity of the fungus.
Ribosomally synthesized and posttranslationally modified peptides (RiPPs) a class of secondary metabolites which are genetically encoded in form of biosynthetic gene clusters (BGCs). These cluster contain modifying enzymes, which allow for a wide spectrum compounds and give the possibility to alter the final product for different purposes. Using antiSMASH we mined the
genomes of 50 different Trichoderma strains across multiple species, looking especially into potential RiPP cluster. The analysis of the Trichoderma genomes predicted nine RiPP BGCs as well as a polyketide-RiPP hybrid cluster in 10 different species. A subsequent analysis using BiG-SCAPE reveals that 7 of these clusters are closely related to each other and the already well-
described ustiloxin-B cluster from Aspergillus flavus. These ustiloxin-like clusters are found exclusively in species belonging to the „Harzianum clade“ and show a very conserved cluster
structure. The three unique cluster posses show no resemblance to the ustiloxin-like cluster and might represent a new class of RiPPs. This is further supported by a multiple sequence alignment
between homologous genes in all 10 cluster. One representative of the ustiloxin-like cluster, as well as the three unique cluster were chosen for further investigation. Using RT-qPCR we determined which genes are co-transcribed under different conditions. In order to complement the transcript analysis a classic proteomics experiment was conducted to confirm the expression of the linked genes. These results underscore the huge biotechnological potential of the genus Trichoderma.
en
dc.description.sponsorship
FWF - Österr. Wissenschaftsfonds
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dc.language.iso
en
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dc.subject
Trichoderma
en
dc.subject
Phylogenomics
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dc.subject
Secondary metabolites
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dc.subject
RiPPs
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dc.subject
ustiloxin-like
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dc.title
Phylogenetic analysis and investigation of RiPP cluster in 50 Trichoderma genomes
en
dc.type
Presentation
en
dc.type
Vortrag
de
dc.relation.grantno
P 34036-B
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dc.type.category
Conference Presentation
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tuw.project.title
Identifizierung und Charakterisierung von RiPPs aus Pilzen
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tuw.researchTopic.id
X1
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tuw.researchTopic.name
Beyond TUW-research foci
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tuw.researchTopic.value
100
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tuw.publication.orgunit
E166-05-1 - Forschungsgruppe Synthetische Biologie und Molekulare Biotechnologie
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tuw.author.orcid
0000-0002-3592-4882
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tuw.event.name
16th European Conference on Fungal Genetics (ECFG16)
en
tuw.event.startdate
05-03-2023
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tuw.event.enddate
08-03-2023
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tuw.event.online
Hybrid
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tuw.event.type
Event for scientific audience
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tuw.event.place
Innsbruck
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tuw.event.country
AT
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tuw.event.institution
Universität Innsbruck
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tuw.event.presenter
Schmal, Matthias
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wb.sciencebranch
Chemische Verfahrenstechnik
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wb.sciencebranch
Biologie
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wb.sciencebranch
Industrielle Biotechnologie
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wb.sciencebranch.oefos
2040
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wb.sciencebranch.oefos
1060
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wb.sciencebranch.oefos
2090
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wb.sciencebranch.value
50
-
wb.sciencebranch.value
35
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wb.sciencebranch.value
15
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item.openairecristype
http://purl.org/coar/resource_type/c_18cp
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item.cerifentitytype
Publications
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item.grantfulltext
none
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item.fulltext
no Fulltext
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item.languageiso639-1
en
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item.openairetype
conference paper not in proceedings
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crisitem.project.funder
FWF - Österr. Wissenschaftsfonds
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crisitem.project.grantno
P 34036-B
-
crisitem.author.dept
E166-05-1 - Forschungsgruppe Synthetische Biologie und Molekulare Biotechnologie
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crisitem.author.orcid
0000-0002-3592-4882
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crisitem.author.parentorg
E166-05 - Forschungsbereich Biochemische Technologie