<div class="csl-bib-body">
<div class="csl-entry">Masi, A. L. (2023, March 7). <i>Mannitol metabolism and osmotic stress answer: a comparative study on Trichoderma reesei and Aureobasidium pullulans</i> [Conference Presentation]. 16th European Conference on Fungal Genetics (ECFG16), Innsbruck, Austria.</div>
</div>
-
dc.identifier.uri
http://hdl.handle.net/20.500.12708/193638
-
dc.description.abstract
Mannitol's role and metabolism in fungi have been widely debated. It has been proposed to serve as carbohydrate storage, neutralize reactive oxygen species, support
germination, and increase resistance to stress conditions such as osmotic stress. But some publications demonstrate that mannitol is less critical for osmotic stress resistance
than glycerol. We hypothesized that the predominance of Page 2 of 3 mannitol or glycerol in the osmotic stress answer might depend on the environmental conditions. We decided to couple this investigation with a clarification of mannitol metabolism. Mannitol metabolism was described as a cycle by Hult and Gatenbeck in 1978. Their proposed cycle, in Alternaria alternata, comprises four enzymes: a mannitol-1- phosphate dehydrogenase, a mannitol-1-phosphatase, a mannitol dehydrogenase, and a hexokinase. Since then, these enzymes have been identified in many fungi, opening the possibility to generate deletion strains for the “mannitol cycle” enzymes to challenge this model. Such tests performed in Aspergillus niger, A. alternata, or Botrytis cinerea demonstrated that the mannitol cycle model could not fully explain mannitol metabolism. Still, no new model could be built from these results. The tests performed vary from one strain to another, making comparison impossible. To clarify the debate, we decided to explore mannitol metabolism by performing the same tests on two fungi: Trichoderma reesei and Aureobasidium pullulans. We aim to generate single and double deletion strains for mannitol-1-phosphate dehydrogenase and mannitol dehydrogenase. Once developed, the strains are cultivated with different carbon sources with or without osmotic stress. During cultivation, polyols and carbon source amounts are monitored. Biomass is measured at the end of the experiment. Our observations will then be confronted with
existing results in other strains to contribute to a new model for mannitol metabolism. These tests will also characterize the osmotic stress answer through polyol production.
en
dc.language.iso
en
-
dc.subject
mannitol
en
dc.subject
fungi
en
dc.subject
Trichoderma
en
dc.subject
metabolism
en
dc.title
Mannitol metabolism and osmotic stress answer: a comparative study on Trichoderma reesei and Aureobasidium pullulans
en
dc.type
Presentation
en
dc.type
Vortrag
de
dc.type.category
Conference Presentation
-
tuw.researchTopic.id
E6
-
tuw.researchTopic.id
X1
-
tuw.researchTopic.name
Sustainable Production and Technologies
-
tuw.researchTopic.name
Beyond TUW-research foci
-
tuw.researchTopic.value
50
-
tuw.researchTopic.value
50
-
tuw.publication.orgunit
E166-05-1 - Forschungsgruppe Synthetische Biologie und Molekulare Biotechnologie
-
tuw.author.orcid
0000-0001-5852-1528
-
tuw.event.name
16th European Conference on Fungal Genetics (ECFG16)
en
tuw.event.startdate
05-03-2023
-
tuw.event.enddate
08-03-2023
-
tuw.event.online
On Site
-
tuw.event.type
Event for scientific audience
-
tuw.event.place
Innsbruck
-
tuw.event.country
AT
-
tuw.event.institution
Universität Innsbruck
-
tuw.event.presenter
Masi, Audrey Laura
-
tuw.event.track
Multi Track
-
wb.sciencebranch
Biologie
-
wb.sciencebranch
Industrielle Biotechnologie
-
wb.sciencebranch.oefos
1060
-
wb.sciencebranch.oefos
2090
-
wb.sciencebranch.value
70
-
wb.sciencebranch.value
30
-
item.languageiso639-1
en
-
item.openairetype
conference paper not in proceedings
-
item.grantfulltext
none
-
item.fulltext
no Fulltext
-
item.cerifentitytype
Publications
-
item.openairecristype
http://purl.org/coar/resource_type/c_18cp
-
crisitem.author.dept
E166-05-1 - Forschungsgruppe Synthetische Biologie und Molekulare Biotechnologie
-
crisitem.author.orcid
0000-0001-5852-1528
-
crisitem.author.parentorg
E166-05 - Forschungsbereich Biochemische Technologie
