<div class="csl-bib-body">
<div class="csl-entry">Ret, D., Gentile Salvatore Alessio, Ammaturo Antonio, Stefentatti, L., Knaus, S., & Untersmayr, E. (2023, February 17). <i>Species specific milk N-Glycosylation</i> [Conference Presentation]. 26th Austrian Carbohydrate Workshop 2023, Austria. http://hdl.handle.net/20.500.12708/193672</div>
</div>
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/193672
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dc.description.abstract
Introduction:
Glycans are key components on cell surfaces and involved in most physiological and pathological processes including cell-cell interaction, antibody receptor binding. Therefore, glycosylation pattern influence inflammation, cancer, autoimmune diseases and infections. Codified in the glycans structure, each nutrient contains a glycosignature depending on the source of the dietary proteins. As glycan introduced by diet were suggested to influence human health based on potential differences with human pattern, we aimed to determine the glycan composition of milk from different sources.
Method
N-glycans are characterized by MALDI mass spectrometry in positive mode after derivatization with methylamine and purification. Total Sialic acids where quantifiedafter labelling with DMBA by HPLC-RP-FL.
Results
The N-glycan structure and the amount of sialic acids detected in human breast milk was compared with infant formula as well as cow, goat, horse and sheep milk. We observed substantial variations in N-glycome depending on the source of the milk samples. The difference between milk from human and non-human source including infant formula was confirm by concentration determination of total sialic acid content. Non-human sialic acid (N-glycolyl neuraminic acid) concentrations were lowest in human breast milk (5 mg/L) compared to infant formula (7 mg/L) and cow’s milk (9 mg/L). For goat and sheep milk higher levels of Neu5Gc that Neu5Ac was detected.
Conclusion
Our result clearly confirm species specific glycan pattern reflected by a distinct N-glycome of different milk samples. As the dietary glycan composition influences human health, immuninutritional effects should be taken into consideration.
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dc.language.iso
en
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dc.subject
glycan
en
dc.title
Species specific milk N-Glycosylation
en
dc.type
Presentation
en
dc.type
Vortrag
de
dc.contributor.affiliation
Medical University of Vienna, Austria
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dc.type.category
Conference Presentation
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tuw.researchTopic.id
M2
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tuw.researchTopic.id
M6
-
tuw.researchTopic.id
M4
-
tuw.researchTopic.name
Materials Characterization
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tuw.researchTopic.name
Biological and Bioactive Materials
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tuw.researchTopic.name
Non-metallic Materials
-
tuw.researchTopic.value
20
-
tuw.researchTopic.value
40
-
tuw.researchTopic.value
40
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tuw.publication.orgunit
E163-02-1 - Forschungsgruppe Polymerchemie und Technologie
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tuw.author.orcid
0000-0002-7461-0923
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tuw.author.orcid
0000-0002-1963-499X
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tuw.event.name
26th Austrian Carbohydrate Workshop 2023
en
tuw.event.startdate
16-02-2023
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tuw.event.enddate
17-02-2023
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tuw.event.online
On Site
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tuw.event.type
Event for scientific audience
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tuw.event.country
AT
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tuw.event.presenter
Ret, Davide
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wb.sciencebranch
Chemie
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wb.sciencebranch
Medizinische Biochemie, Humangenetik
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wb.sciencebranch.oefos
1040
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wb.sciencebranch.oefos
3013
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wb.sciencebranch.value
50
-
wb.sciencebranch.value
50
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item.openairetype
conference paper not in proceedings
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item.languageiso639-1
en
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item.cerifentitytype
Publications
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item.fulltext
no Fulltext
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item.grantfulltext
none
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item.openairecristype
http://purl.org/coar/resource_type/c_18cp
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crisitem.author.dept
E163-02-1 - Forschungsgruppe Polymerchemie und Technologie
-
crisitem.author.dept
Medical University of Vienna
-
crisitem.author.dept
E913 - Betriebsrat (DA) für das wissenschaftliche und künstlerische Universitätspersonal
