<div class="csl-bib-body">
<div class="csl-entry">Weiss, V. (2023, October 18). <i>Gas-phase electrophoresis in vaccine development</i> [Presentation]. Seminar presentation Department of Chemistry 2023, Zagreb, Croatia.</div>
</div>
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/193706
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dc.description.abstract
Gas-phase electrophoresis in vaccine development
Victor U. Weiss
Institute of Chemical Technologies and Analytics, TU Wien, Getreidemarkt 9/164 CTA, 1060 Vienna
Gas-phase electrophoresis applying a nano Electrospray Gas-phase Electrophoretic Mobility Molecular Analyzer (nES GEMMA) instrument will be discussed. Analytes are electrosprayed from a volatile electrolyte solution followed by particle drying and charge-equilibration. This latter step results in mostly neutral yet also a considerable amount of single-charged, dry particles. Subsequently, analytes are separated in the nano Differential Mobility Analyzer (nDMA) part of the nES GEMMA by voltage scanning in a tunable electric field and an orthogonal high sheath flow of particle-free air. As particles are single-charged (neutral species are not regarded), separation of analytes is solely based on their dry particle electrophoretic mobility (EM) diameter. Furthermore, in good accordance with a recommendation of the European Commission for nanoparticle research (2011/696/EU from October 18th, 2011), particle detection is based on the number of particles. Beginning with proteins and protein aggregates nES GEMMA has yielded valuable insights regarding sample purity, analyte stability and aggregational behavior as well as dry particle sizes. Given that a correlation based on molecular weight and EM diameter of well-defined standards can be set up, even the molecular weight of an analyte can be assessed from its EM diameter as first demonstrated for proteins and recently even for intact viruses and virus-like particles. Likewise, liposomes have been measured via gas-phase electrophoresis. Besides the measurement of dry particle size values, the determination of size distributions and the estimation of particle concentrations, analytes of a certain and well-defined size (depending on a fixed applied voltage inside the nDMA) can be collected on various surfaces for orthogonal analyses, for instance atomic force microscopy (AFM), spectroscopic or mass spectrometric measurements. To conclude, nES GEMMA yields valuable insights regarding (bio-)nanoparticle containing samples and can definitely help in vaccine development.
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dc.language.iso
en
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dc.subject
GEMMA
en
dc.subject
Gas-phase electrophoresis
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dc.subject
VLP
en
dc.subject
Vaccines
en
dc.subject
(bio-)nanoparticle
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dc.title
Gas-phase electrophoresis in vaccine development
en
dc.type
Presentation
en
dc.type
Vortrag
de
dc.type.category
Presentation
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tuw.publication.invited
invited
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tuw.researchTopic.id
M2
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tuw.researchTopic.id
M6
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tuw.researchTopic.id
M4
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tuw.researchTopic.name
Materials Characterization
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tuw.researchTopic.name
Biological and Bioactive Materials
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tuw.researchTopic.name
Non-metallic Materials
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tuw.researchTopic.value
40
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tuw.researchTopic.value
40
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tuw.researchTopic.value
20
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tuw.publication.orgunit
E164-01-1 - Forschungsgruppe Massenspektrometrische Bio- und Polymeranalytik
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tuw.author.orcid
0000-0002-0056-6819
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tuw.event.name
Seminar presentation Department of Chemistry 2023
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tuw.event.startdate
18-10-2023
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tuw.event.enddate
18-10-2023
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tuw.event.online
On Site
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tuw.event.type
Event for scientific audience
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tuw.event.place
Zagreb
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tuw.event.country
HR
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tuw.event.presenter
Weiss, Victor
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wb.sciencebranch
Chemie
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wb.sciencebranch.oefos
1040
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wb.sciencebranch.value
100
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item.grantfulltext
none
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item.cerifentitytype
Publications
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item.openairecristype
http://purl.org/coar/resource_type/R60J-J5BD
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item.languageiso639-1
en
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item.openairetype
conference presentation
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item.fulltext
no Fulltext
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crisitem.author.dept
E164-01-1 - Forschungsgruppe Massenspektrometrische Bio- und Polymeranalytik
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crisitem.author.orcid
0000-0002-0056-6819
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crisitem.author.parentorg
E164-01 - Forschungsbereich Imaging und Instrumentelle Analytische Chemie