DC Field
Value
Language
dc.contributor.author
Kolarević, Stoimir
-
dc.contributor.author
Micsinai, Adrienn
-
dc.contributor.author
Szántó-Egész, Réka
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dc.contributor.author
Lukács, Alena
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dc.contributor.author
Kračun-Kolarević, Margareta
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dc.contributor.author
Djordjevic, Ana
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dc.contributor.author
Vojnović Milutinović, Danijela
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dc.contributor.author
Jovanović Marić, Jovana
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dc.contributor.author
Kirschner, Alexander K.T.
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dc.contributor.author
Farnleitner, Andreas
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dc.contributor.author
Linke, Rita Barbara
-
dc.contributor.author
Đukic, Aleksandar
-
dc.contributor.author
Kostic-Vukovic, Jovana
-
dc.contributor.author
Paunović, Momir
-
dc.date.accessioned
2024-02-20T08:37:48Z
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dc.date.available
2024-02-20T08:37:48Z
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dc.date.issued
2022-10-15
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dc.identifier.citation
<div class="csl-bib-body">
<div class="csl-entry">Kolarević, S., Micsinai, A., Szántó-Egész, R., Lukács, A., Kračun-Kolarević, M., Djordjevic, A., Vojnović Milutinović, D., Jovanović Marić, J., Kirschner, A. K. T., Farnleitner, A., Linke, R. B., Đukic, A., Kostic-Vukovic, J., & Paunović, M. (2022). Wastewater-based epidemiology in countries with poor wastewater treatment - Epidemiological indicator function of SARS-CoV-2 RNA in surface waters. <i>Science of the Total Environment</i>, <i>843</i>, Article 156964. https://doi.org/10.1016/j.scitotenv.2022.156964</div>
</div>
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dc.identifier.issn
0048-9697
-
dc.identifier.uri
http://hdl.handle.net/20.500.12708/194206
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dc.description.abstract
Wastewater-based epidemiology (WBE) surveillance of COVID-19 and other future outbreaks is a challenge for developing countries as most households are not connected to a sewerage system. In December 2019, SARS-CoV-2 RNA was detected in the Danube River at a site severely affected by wastewaters from Belgrade. Rivers are much more complex systems than wastewater systems, and efforts are needed to address all the factors influencing the adoption of WBE as an alternative to targeting raw wastewater. Our objective was to provide a more detailed insight into the potential of SARS-CoV-2 surveillance in Serbian surface waters for epidemiological purposes. Water samples were collected at 12 sites along the Sava and Danube rivers in Belgrade during the fourth COVID-19 wave in Serbia that started in late February 2021. RNA was concentrated using Amicon Ultra-15 centrifugal filters and quantified using RT-qPCR with primer sets targeting nucleocapsid (N1 and N2) and envelope (E) protein genes. Microbiological (faecal indicator bacteria and human and animal genetic faecal source tracking markers), epidemiological, physicochemical and hydromorphological parameters were analysed in parallel. From 44 samples, SARS-CoV-2 RNA was detected in 31, but only at 4 concentrations above the level of quantification (ranging from 8.47 × 103 to 2.07 × 104 gc/L). The results indicated that surveillance of SARS-CoV-2 RNA in surface waters as ultimate recipients could be used as an epidemiological early-warning tool in countries lacking wastewater treatment and proper sewerage infrastructure. The performance of the applied approach, including advanced sampling site characterization to trace and identify sites with significant raw sewage influence from human populations, could be further improved by adaptation of the methodology for processing higher volumes of samples and enrichment factors, which should provide the quantitative instead of qualitative data needed for WBE.
en
dc.language.iso
en
-
dc.publisher
ELSEVIER
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dc.relation.ispartof
Science of the Total Environment
-
dc.subject
Humans
en
dc.subject
RNA, Viral
en
dc.subject
SARS-CoV-2
en
dc.subject
Wastewater
en
dc.subject
Wastewater-Based Epidemiological Monitoring
en
dc.subject
Faecal pollution
en
dc.subject
SARS-CoV-2
en
dc.subject
Serbia
en
dc.subject
Surface waters
en
dc.subject
Untreated wastewaters
en
dc.subject
COVID-19
en
dc.subject
Water Purification
en
dc.title
Wastewater-based epidemiology in countries with poor wastewater treatment - Epidemiological indicator function of SARS-CoV-2 RNA in surface waters
en
dc.type
Article
en
dc.type
Artikel
de
dc.identifier.url
https://api.elsevier.com/content/abstract/scopus_id/85133165033
-
dc.contributor.affiliation
University of Belgrade, Serbia
-
dc.contributor.affiliation
Wessling (Hungary), Hungary
-
dc.contributor.affiliation
Biomi Ltd., Hungary
-
dc.contributor.affiliation
Biomi Ltd., Hungary
-
dc.contributor.affiliation
University of Belgrade, Serbia
-
dc.contributor.affiliation
University of Belgrade, Serbia
-
dc.contributor.affiliation
University of Belgrade, Serbia
-
dc.contributor.affiliation
University of Belgrade, Serbia
-
dc.contributor.affiliation
Medical University of Vienna, Austria
-
dc.contributor.affiliation
University of Belgrade, Serbia
-
dc.contributor.affiliation
University of Belgrade, Serbia
-
dc.contributor.affiliation
University of Belgrade, Serbia
-
dc.type.category
Original Research Article
-
tuw.container.volume
843
-
tuw.journal.peerreviewed
true
-
tuw.peerreviewed
true
-
wb.publication.intCoWork
International Co-publication
-
tuw.researchTopic.id
E4
-
tuw.researchTopic.name
Environmental Monitoring and Climate Adaptation
-
tuw.researchTopic.value
100
-
dcterms.isPartOf.title
Science of the Total Environment
-
tuw.publication.orgunit
E166-05-3 - Forschungsgruppe Mikrobiologie und Molekulare Diagnostik
-
tuw.publication.orgunit
E057-08 - Fachbereich Forschungszentrum Wasser und Gesundheit
-
tuw.publisher.doi
10.1016/j.scitotenv.2022.156964
-
dc.identifier.articleid
156964
-
dc.identifier.eissn
1879-1026
-
tuw.author.orcid
0000-0002-6938-8803
-
tuw.author.orcid
0000-0001-5745-4589
-
tuw.author.orcid
0000-0002-8885-2456
-
tuw.author.orcid
0000-0002-5691-2119
-
tuw.author.orcid
0000-0002-2584-5048
-
tuw.author.orcid
0000-0003-1635-7787
-
tuw.author.orcid
0000-0002-9797-3073
-
tuw.author.orcid
0000-0002-6277-7048
-
tuw.author.orcid
0000-0003-2474-3223
-
tuw.author.orcid
0000-0002-6432-1191
-
dc.description.sponsorshipexternal
Fond für Wissenschaftliche Forschung
-
dc.relation.grantnoexternal
P32464
-
wb.sci
true
-
wb.sciencebranch
Biologie
-
wb.sciencebranch
Sonstige Medizinisch-theoretische Wissenschaften
-
wb.sciencebranch.oefos
1060
-
wb.sciencebranch.oefos
3019
-
wb.sciencebranch.value
50
-
wb.sciencebranch.value
50
-
item.fulltext
no Fulltext
-
item.openairetype
research article
-
item.languageiso639-1
en
-
item.grantfulltext
none
-
item.openairecristype
http://purl.org/coar/resource_type/c_2df8fbb1
-
item.cerifentitytype
Publications
-
crisitem.author.dept
University of Belgrade
-
crisitem.author.dept
Wessling (Hungary)
-
crisitem.author.dept
Biomi Ltd., Hungary
-
crisitem.author.dept
Biomi Ltd., Hungary
-
crisitem.author.dept
University of Belgrade
-
crisitem.author.dept
University of Belgrade
-
crisitem.author.dept
University of Belgrade
-
crisitem.author.dept
University of Belgrade
-
crisitem.author.dept
Medical University of Vienna
-
crisitem.author.dept
E166-05-3 - Forschungsgruppe Umweltmikrobiologie and Molekulare Diagnostik
-
crisitem.author.dept
E166-05-3 - Forschungsgruppe Umweltmikrobiologie and Molekulare Diagnostik
-
crisitem.author.dept
University of Belgrade
-
crisitem.author.dept
University of Belgrade
-
crisitem.author.dept
University of Belgrade
-
crisitem.author.orcid
0000-0002-6938-8803
-
crisitem.author.orcid
0000-0001-5745-4589
-
crisitem.author.orcid
0000-0002-5691-2119
-
crisitem.author.orcid
0000-0002-2584-5048
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crisitem.author.orcid
0000-0003-1635-7787
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crisitem.author.orcid
0000-0002-6277-7048
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crisitem.author.orcid
0000-0002-6432-1191
-
crisitem.author.parentorg
E166-05 - Forschungsbereich Biochemische Technologie
-
crisitem.author.parentorg
E166-05 - Forschungsbereich Biochemische Technologie
-
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