<div class="csl-bib-body">
<div class="csl-entry">Hochenegger, N., Mach, R., Mach-Aigner, A., & Zimmermann, C. (2023, March 7). <i>On the interaction of SREBP Sah2 and the pleiotropic regulator Xpp1 in Trichoderma reesei</i> [Poster Presentation]. 16th European Conference on Fungal Genetics, Innsbruck, Austria.</div>
</div>
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/195045
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dc.description.abstract
Sterol regulatory element binding proteins (SREBPs) are transcription factors that are conserved from yeast to humans. In mammals they regulate cholesterol synthesis, in fungi they are additionally involved in hypoxic response (Sre1 in Schizosaccharomyces pombe), resistance to azole compounds (Sre1 in Cryptococcus neoformans), and pathogenesis (SrbA in Aspergillus fumigatus), among others. More recent studies also reveal the role of SREBP Sah2 in protein secretion in Neurospora crassa.
Typically, the N-terminus of SREBPs harbors the transcription factor domain, which is a basic-helix-loop-helix leucine zipper (bHLH-LZ) structure. Thereby, the leucine zipper allows for dimerization. Structure analysis of Sah2 of Trichoderma ressei revealed, that its bHLH-LZ is highly similar to the C-terminal bHLH-LZ of transcription factor Xpp1.Our recent work elucidated the critical role of Xpp1 in the metabolism of T. reesei. When Xpp1 is deleted, growth is impaired and the expression of sorbicillinoids and low weight molecules is increased. Those findings support the conclusions from RNA-Seq results, that Xpp1 activates primary metabolism, however, it represses secondary metabolism.
In this work, we investigated the interaction between Sah2 and Xpp1 in order to regulate metabolic processes in T. reesei. Different imaging techniques were used to observe the co-localisation of the fluorescence-tagged molecules inside the cell. Additionally, we analyzed different metabolic processes and their dependencies from the presence of either transcription factor.
en
dc.language.iso
en
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dc.subject
Trichoderma reesei
en
dc.subject
secondary metabolism
en
dc.subject
sterol regulatory element binding protein
en
dc.subject
fluorescence
en
dc.title
On the interaction of SREBP Sah2 and the pleiotropic regulator Xpp1 in Trichoderma reesei
en
dc.type
Presentation
en
dc.type
Vortrag
de
dc.type.category
Poster Presentation
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tuw.researchTopic.id
X1
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tuw.researchTopic.name
Beyond TUW-research foci
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tuw.researchTopic.value
100
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tuw.publication.orgunit
E166-05-1 - Forschungsgruppe Synthetische Biologie und Molekulare Biotechnologie
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tuw.author.orcid
0000-0001-8663-0705
-
tuw.author.orcid
0000-0003-2375-7244
-
tuw.author.orcid
0000-0002-7575-2317
-
tuw.author.orcid
0000-0002-6440-2100
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tuw.event.name
16th European Conference on Fungal Genetics
en
tuw.event.startdate
05-03-2023
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tuw.event.enddate
08-03-2023
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tuw.event.online
On Site
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tuw.event.type
Event for scientific audience
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tuw.event.place
Innsbruck
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tuw.event.country
AT
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tuw.event.presenter
Hochenegger, Nadine
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wb.sciencebranch
Biologie
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wb.sciencebranch.oefos
1060
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wb.sciencebranch.value
100
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item.fulltext
no Fulltext
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item.grantfulltext
none
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item.languageiso639-1
en
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item.openairetype
conference poster not in proceedings
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item.cerifentitytype
Publications
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item.openairecristype
http://purl.org/coar/resource_type/c_18co
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crisitem.author.dept
E166-05-1 - Forschungsgruppe Synthetische Biologie und Molekulare Biotechnologie
-
crisitem.author.dept
E166 - Institut für Verfahrenstechnik, Umwelttechnik und technische Biowissenschaften
-
crisitem.author.dept
E166-05 - Forschungsbereich Biochemische Technologie
-
crisitem.author.dept
E166-05-1 - Forschungsgruppe Synthetische Biologie und Molekulare Biotechnologie
-
crisitem.author.orcid
0000-0001-8663-0705
-
crisitem.author.orcid
0000-0003-2375-7244
-
crisitem.author.orcid
0000-0002-7575-2317
-
crisitem.author.orcid
0000-0002-6440-2100
-
crisitem.author.parentorg
E166-05 - Forschungsbereich Biochemische Technologie
-
crisitem.author.parentorg
E150 - Fakultät für Technische Chemie
-
crisitem.author.parentorg
E166 - Institut für Verfahrenstechnik, Umwelttechnik und technische Biowissenschaften
-
crisitem.author.parentorg
E166-05 - Forschungsbereich Biochemische Technologie