<div class="csl-bib-body">
<div class="csl-entry">Eggenreich, B., Rajamanickam, V., Wurm, D. J., Fricke, J., Herwig, C., & Spadiut, O. (2017). A combination of HPLC and automated data analysis for monitoring the efficiency of high-pressure homogenization. <i>Microbial Cell Factories</i>, <i>16</i>, 1–10. https://doi.org/10.1186/s12934-017-0749-y</div>
</div>
-
dc.identifier.issn
1475-2859
-
dc.identifier.uri
http://hdl.handle.net/20.500.12708/19857
-
dc.description.abstract
Cell disruption is a key unit operation to make valuable, intracellular target products accessible for further downstream unit operations. Independent of the applied cell disruption method, each cell disruption process must be evaluated with respect to disruption efficiency and potential product loss. Current state-of-the-art methods, like measuring the total amount of released protein and plating-out assays, are usually time-delayed and involve manual intervention making them error-prone. An automated method to monitor cell disruption efficiency at-line is not available to date.
en
dc.language.iso
en
-
dc.publisher
BMC
-
dc.relation.ispartof
Microbial Cell Factories
-
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
-
dc.subject
Cell disruption
en
dc.subject
data analysis
en
dc.subject
E.Coli
en
dc.subject
HPLC
en
dc.subject
High-pressure homogenization
en
dc.title
A combination of HPLC and automated data analysis for monitoring the efficiency of high-pressure homogenization