Converting a symmetrical Gaussian beam into a thin tunable light sheet

Abstract

In this study, we investigate the performance of axial-conical lenses, commonly referred to as Powell lenses, featuring varying fan angles of 5°, 7.5°, 10°, 15°, and 20°. Our objective is to evaluate their suitability for designing a light sheet generator tailored for fluorescence light-sheet microscopy of large samples. Our results indicate that Powell lenses with fan angles of 5° and 7.5° when integrated with additional aspheric components, exhibit optimal characteristics for this application. Specifically, employing a Powell lens with a 7.5° fan angle and 0.2 mm roundness at the tip facilitates the generation of a light sheet ideal for illuminating samples within a size range of 2,000 µm–15,000 µm. To validate the practicality of our optical design for real-world imaging tasks, we conducted imaging experiments on chicken embryos aged between 3 and 7 days. Our light-sheet microscopy system successfully captured intricate structural details, particularly highlighting the ongoing differentiation of the inner anatomy of these specimens. This approach has a high potential to improve the screening of pharmaceutical drugs acting on the vascularization of the chorioallantois membrane (CAM), a technique that is widely used in pharmaceutical research.